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Contribution of cell elongation to the biofilm formation of Pseudomonas aeruginosa during anaerobic respiration

Title
 Contribution of cell elongation to the biofilm formation of Pseudomonas aeruginosa during anaerobic respiration 
Authors
 Mi Young Yoon ; Kang-Mu Lee ; Sang Sun Yoon ; Yongjin Park 
Issue Date
2011
Journal Title
 PLoS One 
ISSN
 1932-6203 
Citation
 PLoS One, Vol.6(1) : e16105, 2011 
Abstract
Pseudomonas aeruginosa, a gram-negative bacterium of clinical importance, forms more robust biofilm during anaerobic respiration, a mode of growth presumed to occur in abnormally thickened mucus layer lining the cystic fibrosis (CF) patient airway. However, molecular basis behind this anaerobiosis-triggered robust biofilm formation is not clearly defined yet. Here, we identified a morphological change naturally accompanied by anaerobic respiration in P. aeruginosa and investigated its effect on the biofilm formation in vitro. A standard laboratory strain, PAO1 was highly elongated during anaerobic respiration compared with bacteria grown aerobically. Microscopic analysis demonstrated that cell elongation likely occurred as a consequence of defective cell division. Cell elongation was dependent on the presence of nitrite reductase (NIR) that reduces nitrite (NO(2) (-)) to nitric oxide (NO) and was repressed in PAO1 in the presence of carboxy-PTIO, a NO antagonist, demonstrating that cell elongation involves a process to respond to NO, a spontaneous byproduct of the anaerobic respiration. Importantly, the non-elongated NIR-deficient mutant failed to form biofilm, while a mutant of nitrate reductase (NAR) and wild type PAO1, both of which were highly elongated, formed robust biofilm. Taken together, our data reveal a role of previously undescribed cell biological event in P. aeruginosa biofilm formation and suggest NIR as a key player involved in such process.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/92564
DOI
10.1371/journal.pone.0016105
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Microbiology
Yonsei Authors
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