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Down-regulation of Bcl-2 is mediated by NF-κB activation in Helicobacter pylori-induced apoptosis of gastric epithelial cells

Title
Down-regulation of Bcl-2 is mediated by NF-κB activation in Helicobacter pylori-induced apoptosis of gastric epithelial cells
Authors
Sang Hui Chu;Joo Weon Lim;Hyeyoung Kim;Kyung Hwan Kim;Eung-Seok Lee;Dong Goo Kim
Issue Date
2011
Journal Title
Scandinavian Journal of Gastroenterology
ISSN
0036-5521
Citation
Scandinavian Journal of Gastroenterology, Vol.46(2) : 148~155, 2011
Abstract
OBJECTIVE: Bcl-2 family is involved in the regulation of apoptosis. NF-κB activation is associated with either the expression of Bcl-2 or down-regulation of Bcl-2 depending on cell types and stimuli. Previously, we showed NF-κB activation, decrease in the level of Bcl-2, and apoptosis in Helicobacter pylori (H. pylori)-infected gastric epithelial cells. The present study aims to investigate the relation of Bcl-2 expression and NF-κB activation in H. pylori-induced apoptotic cell death of AGS (gastric adenocarcinoma) cells. MATERIAL AND METHODS: AGS cells were transfected with mutant IκBα to suppress NF-κB activation or Bcl-2 gene to induce overexpression of Bcl-2. mRNA expression of Bcl-2, p53 and Bax, DNA fragmentation, cell viability, and the numbers of apoptotic cells were determined. RESULTS: H. pylori induced decrease in Bcl-2, but increase in p53 and Bax at the levels of mRNA and protein in AGS cells. H. pylori-induced increment of apoptotic cells and decrease in Bcl-2 level were inhibited in the cells transfected with mutant IκBα gene as compared with the cells transfected with control vector. H. pylori-induced apoptosis determined by apoptotic cells, DNA fragmentation, and cell viability was inhibited in the cells transfected with Bcl-2 gene. CONCLUSION: Down-regulation of Bcl-2 is mediated by NF-κB activation, which may be the underlying mechanism of apoptosis in H. pylori-infected gastric epithelial cells.
URI
http://informahealthcare.com/doi/abs/10.3109/00365521.2010.525255

http://ir.ymlib.yonsei.ac.kr/handle/22282913/92540
DOI
10.3109/00365521.2010.525255
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Pharmacology
1. 연구논문 > 3. College of Nursing > Dept. of Nursing Environment Systems
Yonsei Authors
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