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Characterization of different subpopulations from bone marrow-derived mesenchymal stromal cells by alkaline phosphatase expression
DC Field | Value | Language |
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dc.contributor.author | 김현옥 | - |
dc.contributor.author | 윤동석 | - |
dc.contributor.author | 이진우 | - |
dc.date.accessioned | 2014-12-19T17:41:23Z | - |
dc.date.available | 2014-12-19T17:41:23Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 1547-3287 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/91868 | - |
dc.description.abstract | Multiple surface markers have been utilized for the enrichment of bone marrow mesenchymal stromal cells (MSCs) and to define primitive stem cells. We classified human bone marrow-derived MSC populations according to tissue nonspecific alkaline phosphatase (TNAP) activity. TNAP expression varied among unexpanded primary MSCs, and its level was not related to colony-forming activity or putative surface markers, such as CD105 and CD29, donor age, or gender. TNAP levels were increased in larger cells, and a colony-forming unit-fibroblast assay revealed that the colony size was decreased during in vitro expansion. TNAP-positive (TNAP+) MSCs showed limited multipotential capacity, whereas TNAP-negative (TNAP-) MSCs retained the differentiation potential into 3 lineages (osteogenic-, adipogenic-, and chondrogenic differentiation). High degree of calcium mineralization and high level of osteogenic-related gene expression (osteopontin, dlx5, and cbfa1) were found in TNAP+ cells. In contrast, during chondrogenic differentiation, type II collagen was successfully induced in TNAP- cells, but not in TNAP+ cells. TNAP+ cells showed high levels of the hypertrophic markers, type X collagen and cbfa1. Mesenchymal stem cell antigen-1 (MSCA-1) is identical to TNAP. Therefore, TNAP+ cells were sorted by using antibody targeting MSCA-1. MSCA-1-positive cells sorted for TNAP+ cells exhibited low proliferation rates. Expression of cell cycle-related genes (cyclin A2, CDK2, and CDK4) and pluripotency marker genes (rex1 and nanog) were higher in TNAP- MSC than in TNAP+ MSC. Therefore, TNAP- cells can be described as more primitive bone marrow-derived cells and TNAP levels in MSCs can be used to predict chondrocyte hypertrophy or osteogenic capacity. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | STEM CELLS AND DEVELOPMENT | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adipocytes/cytology | - |
dc.subject.MESH | Adipogenesis/genetics | - |
dc.subject.MESH | Adult | - |
dc.subject.MESH | Aged | - |
dc.subject.MESH | Alkaline Phosphatase/metabolism* | - |
dc.subject.MESH | Biomarkers/metabolism | - |
dc.subject.MESH | Cell Cycle/genetics | - |
dc.subject.MESH | Cell Lineage/genetics | - |
dc.subject.MESH | Cell Proliferation | - |
dc.subject.MESH | Cell Size | - |
dc.subject.MESH | Chondrogenesis/genetics | - |
dc.subject.MESH | Clone Cells | - |
dc.subject.MESH | Colony-Forming Units Assay | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Fetal Blood/cytology | - |
dc.subject.MESH | Flow Cytometry | - |
dc.subject.MESH | Gene Expression Regulation | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Mesenchymal Stromal Cells/cytology* | - |
dc.subject.MESH | Mesenchymal Stromal Cells/enzymology* | - |
dc.subject.MESH | Middle Aged | - |
dc.subject.MESH | Osteoblasts/cytology | - |
dc.subject.MESH | Osteogenesis/genetics | - |
dc.subject.MESH | Pluripotent Stem Cells/cytology | - |
dc.subject.MESH | Pluripotent Stem Cells/metabolism | - |
dc.subject.MESH | Tissue Donors | - |
dc.subject.MESH | Young Adult | - |
dc.title | Characterization of different subpopulations from bone marrow-derived mesenchymal stromal cells by alkaline phosphatase expression | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Orthopedic Surgery (정형외과학) | - |
dc.contributor.googleauthor | Yun Hee Kim | - |
dc.contributor.googleauthor | Dong Suk Yoon | - |
dc.contributor.googleauthor | Hyun Ok Kim | - |
dc.contributor.googleauthor | Jin Woo Lee | - |
dc.identifier.doi | 22702738 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01122 | - |
dc.contributor.localId | A02547 | - |
dc.contributor.localId | A03230 | - |
dc.relation.journalcode | J02684 | - |
dc.identifier.eissn | 1557-8534 | - |
dc.identifier.pmid | 22702738 | - |
dc.identifier.url | http://online.liebertpub.com/doi/abs/10.1089/scd.2011.0349 | - |
dc.subject.keyword | Adipocytes/cytology | - |
dc.subject.keyword | Adipogenesis/genetics | - |
dc.subject.keyword | Adult | - |
dc.subject.keyword | Aged | - |
dc.subject.keyword | Alkaline Phosphatase/metabolism* | - |
dc.subject.keyword | Biomarkers/metabolism | - |
dc.subject.keyword | Cell Cycle/genetics | - |
dc.subject.keyword | Cell Lineage/genetics | - |
dc.subject.keyword | Cell Proliferation | - |
dc.subject.keyword | Cell Size | - |
dc.subject.keyword | Chondrogenesis/genetics | - |
dc.subject.keyword | Clone Cells | - |
dc.subject.keyword | Colony-Forming Units Assay | - |
dc.subject.keyword | Female | - |
dc.subject.keyword | Fetal Blood/cytology | - |
dc.subject.keyword | Flow Cytometry | - |
dc.subject.keyword | Gene Expression Regulation | - |
dc.subject.keyword | Humans | - |
dc.subject.keyword | Male | - |
dc.subject.keyword | Mesenchymal Stromal Cells/cytology* | - |
dc.subject.keyword | Mesenchymal Stromal Cells/enzymology* | - |
dc.subject.keyword | Middle Aged | - |
dc.subject.keyword | Osteoblasts/cytology | - |
dc.subject.keyword | Osteogenesis/genetics | - |
dc.subject.keyword | Pluripotent Stem Cells/cytology | - |
dc.subject.keyword | Pluripotent Stem Cells/metabolism | - |
dc.subject.keyword | Tissue Donors | - |
dc.subject.keyword | Young Adult | - |
dc.contributor.alternativeName | Kim, Hyun Ok | - |
dc.contributor.alternativeName | Yoon, Dong Suk | - |
dc.contributor.alternativeName | Lee, Jin Woo | - |
dc.contributor.affiliatedAuthor | Kim, Hyun Ok | - |
dc.contributor.affiliatedAuthor | Yoon, Dong Suk | - |
dc.contributor.affiliatedAuthor | Lee, Jin Woo | - |
dc.citation.volume | 21 | - |
dc.citation.number | 16 | - |
dc.citation.startPage | 2956 | - |
dc.citation.endPage | 2968 | - |
dc.identifier.bibliographicCitation | STEM CELLS AND DEVELOPMENT, Vol.21(16) : 2956-2968, 2012 | - |
dc.identifier.rimsid | 29973 | - |
dc.type.rims | ART | - |
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