Elevated interleukin-32 expression in granulomatosis with polyangiitis
Suyoung Bae ; Yong-Gil Kim ; Soohyun Kim ; Eun Young Song ; Yong-Beom Park ; Bin Yoo ; Chang-Keun Lee ; Areum Kwak ; Eusom Kim ; Kwangwon Hong ; Hyunjhung Jeon ; Taebong Kang ; Siyoung Lee ; Jaewoo Hong ; Jida Choi
Rheumatology, Vol.51(11) : 1979~1988, 2012
To evaluate the role of IL-32 in granulomatosis with polyangiitis (GPA) patients and the relationship between IL-32 and disease activity, as PR3 has the ability to bind and activate IL-32, which has been described as a novel cytokine that induces inflammatory cytokines.
We investigated the level of IL-32, PR3, TNF-α and IL-6 in GPA patients by using ELISA. Northern blot was used to analyse the level of IL-32 mRNA in leucocytes of GPA patients. The intracellular colocalization of IL-32 and PR3 in leucocytes was examined by IF staining.
We observed that IL-32 and PR3 levels in GPA patients were increased significantly when compared with normal individuals and each was tightly associated (P < 0.001). Northern blot analysis revealed that the mRNA level of IL-32 was prominently elevated in leucocytes of GPA patients. The intracellular colocalization of IL-32 and PR3 in leucocytes from GPA patients vs normal individuals was verified by IF staining.
IL-32 level was elevated in GPA patients but its level was changed by treatment response. IL-32 could be an index in GPA and play a role in the aetiology of GPA.