Osteoinductive activity of biphasic calcium phosphate with different rhBMP-2 doses in rats
Ji-Woong Jang ; Jeong-Ho Yun ; Kyoo-Sung Cho ; Seong-Ho Choi ; Chang-Sung Kim ; Ui-Won Jung ; Ju-Woong Jang ; Kwang-Il Lee
Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology, Vol.113(4) : 480~487, 2012
Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology
OBJECTIVE: The aim of the current study was to determine whether a hydroxyapatite (HA)/beta-tricalcium phosphate (β-TCP) ratio of 20/80 impregnated with recombinant human bone morphogenetic protein (rhBMP-2) enhances new bone formation and to evaluate the dose-dependent response of rhBMP-2.
STUDY DESIGN: Critical-sized calvarial defects were made in rats, and biphasic calcium phosphate (BCP) with different rhBMP-2 doses was loaded into rat calvarial defects. The animals were allowed to heal for either 2 or 8 weeks.
RESULTS: The percentages of new bone after 2 and 8 weeks of healing were significantly greater in the rhBMP-2-treated groups (at all doses) than in the control groups. The percentage of remaining BCP was significantly lower at 8 weeks than at 2 weeks in all groups that included BCP.
CONCLUSIONS: rhBMP-2 administered using a BCP carrier significantly induces new bone formation. A dose-dependent response was not shown in the present study.