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Differentially regulated functional gene clusters identified in early hypoxic cardiomyocytes.

Authors
 함온주 ; 황기철 ; 박준희 ; 송병욱 ; 이세연 ; 이창연 ; 차민지 ; 최은미 
Citation
 Molecular Biology Reports, Vol.39(10) : 9549~9556, 2012 
Journal Title
 Molecular Biology Reports 
ISSN
 0301-4851 
Issue Date
2012
Abstract
Pathological stress including myocardial infarction and hypertension causes a negative effect on calcium regulation and homeostasis. Nevertheless, few studies reveal that Ca(2+) regulatory genes are related to pathological status in cardiomyocytes under early hypoxia. To determine the alteration of Ca(2+)-related gene in hypoxic myocytes, primary neonatal rat ventricular cardiomyocytes (NRVCMs) was isolated. Survival of hypoxic NRVCMs was significantly decreased in 6 h. We confirmed an increase of reactive oxygen species (ROS) generation and Ca(2+) overload in hypoxic NRVCMs by using 2',7'-dichlorodihydro-fluorescein diacetate (H2DCFDA) and FACS analysis. Furthermore, survival/apoptotic signals were also regulated in same condition. The expression profiles of more than 30,000 genes from NRVCMs that were subjected to early hypoxia revealed 630 genes that were differentially regulated. The intracellular Na(+) overload and Ca(2+) handling genes with at least two-fold changes were confirmed. The levels of Ca(2+)-handling proteins (calsequestrin, calmodulin, and calreticulin), ion channels (NCX, Na(+)-K(+)-ATPase, SERCA2a, and PLB), and stress markers (RyR2, ANP, and BNP) were significantly altered in early hypoxia. These results demonstrate that early hypoxia alters Ca(2+)-related gene expression in NRVCMs, leading to pathological status.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/91465
DOI
10.1007/s11033-012-1819-1
Appears in Collections:
1. 연구논문 > 5. Research Institutes > Yonsei Cardiovascular Research Institute
1. 연구논문 > 1. College of Medicine > Yonsei Biomedical Research Center
1. 연구논문 > 1. College of Medicine > Dept. of Life Science
Yonsei Authors
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Link
 http://link.springer.com/article/10.1007%2Fs11033-012-1819-1
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