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A lectin-coupled, targeted proteomic mass spectrometry (MRM MS) platform for identification of multiple liver cancer biomarkers in human plasma

Authors
 Yeong Hee Ahn ; Park Min Shin ; Jong Shin Yoo ; Hoguen Kim ; Gun Wook Park ; Na Ree Oh 
Citation
 Journal of Proteomics, Vol.75(17) : 5507~5515, 2012 
Journal Title
 Journal of Proteomics 
ISSN
 1874-3919 
Issue Date
2012
Abstract
Aberrantly glycosylated proteins related to liver cancer progression were captured with specific lectin and identified from human plasma by multiple reaction monitoring (MRM) mass spectrometry as multiple biomarkers for hepatocellular carcinoma (HCC). The lectin fractionation for fucosylated protein glycoforms in human plasma was conducted with a fucose-specific aleuria aurantia lectin (AAL). Following tryptic digestion of the lectin-captured fraction, plasma samples from 30 control cases (including 10 healthy, 10 hepatitis B virus [HBV], and 10 cirrhosis cases) and 10 HCC cases were quantitatively analyzed by MRM to identify which glycoproteins are viable HCC biomarkers. A1AG1, AACT, A1AT, and CERU were found to be potent biomarkers to differentiate HCC plasma from control plasmas. The AUROC generated independently from these four biomarker candidates ranged from 0.73 to 0.92. However, the lectin-coupled MRM assay with multiple combinations of biomarker candidates is superior statistically to those generated from the individual candidates with AUROC more than 0.95, which can be an alternative to the immunoassay inevitably requiring tedious development of multiple antibodies against biomarker candidates to be verified. Eventually the lectin-coupled, targeted proteomic mass spectrometry (MRM MS) platform was found to be efficient to identify multiple biomarkers from human plasma according to cancer progression.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/91077
DOI
10.1016/j.jprot.2012.06.027
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Pathology
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Link
 http://www.sciencedirect.com/science/article/pii/S1874391912004940
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