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Proteomic analysis of microvesicles derived from human mesenchymal stem cells

Title
 Proteomic analysis of microvesicles derived from human mesenchymal stem cells 
Authors
 Han-Soo Kim ; Do-Young Choi ; Dong-Wook Kim ; Kwang Pyo Kim ; Daehee Hwang ; Jin Woo Jung ; Jeong Won Kang ; Seong-Mi Choi ; So Jeong Yun 
Issue Date
2012
Journal Title
 Journal of Proteome Research 
ISSN
 1535-3893 
Citation
 Journal of Proteome Research, Vol.11(2) : 839~849, 2012 
Abstract
Mesenchymal stem cells (MSCs) have emerged as a promising means for treating degenerative or incurable diseases. Recent studies have shown that microvesicles (MVs) from MSCs (MSC-MVs) contribute to recovery of damaged tissues in animal disease models. Here, we profiled the MSC-MV proteome to investigate their therapeutic effects. LC-MS/MS analysis of MSC-MVs identified 730 MV proteins. The MSC-MV proteome included five positive and two variable known markers of MSCs, but no negative marker, as well as 43 surface receptors and signaling molecules controlling self-renewal and differentiation of MSCs. Functional enrichment analysis showed that cellular processes represented by the MSC-MV proteins include cell proliferation, adhesion, migration, and morphogenesis. Integration of MSC's self-renewal and differentiation-related genes and the proteome of MSC-conditioned media (MSC-CM) with the MSC-MV proteome revealed potential MV protein candidates that can be associated with the therapeutic effects of MSC-MVs: (1) surface receptors (PDGFRB, EGFR, and PLAUR); (2) signaling molecules (RRAS/NRAS, MAPK1, GNA13/GNG12, CDC42, and VAV2); (3) cell adhesion (FN1, EZR, IQGAP1, CD47, integrins, and LGALS1/LGALS3); and (4) MSC-associated antigens (CD9, CD63, CD81, CD109, CD151, CD248, and CD276). Therefore, the MSC-MV proteome provides a comprehensive basis for understanding the potential of MSC-MVs to affect tissue repair and regeneration.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/91070
DOI
10.1021/pr200682z
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Laboratory Medicine
1. 연구논문 > 1. College of Medicine > Yonsei Biomedical Research Center
1. 연구논문 > 1. College of Medicine > Dept. of Physiology
Yonsei Authors
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Link
 http://pubs.acs.org/doi/abs/10.1021/pr200682z
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