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Chromosomal cephalosporinase in Enterobacter hormaechei as an ancestor of ACT-1 plasmid-mediated AmpC β-lactamase

Title
 Chromosomal cephalosporinase in Enterobacter hormaechei as an ancestor of ACT-1 plasmid-mediated AmpC β-lactamase 
Authors
 Kyoung Ho Roh ; Wonkeun Song ; Yunsop Chong ; Kyungwon Lee ; Dongeun Yong ; Jong Sik Chun ; Ha Na Yi ; Jong Hwa Yum ; Yang Soon Lee ; Hae-Sun Chung 
Issue Date
2012
Journal Title
 Journal of Medical Microbiology 
ISSN
 0022-2615 
Citation
 Journal of Medical Microbiology, Vol.61(pt 1) : 94~100, 2012 
Abstract
In this study of the diversity of AmpC β-lactamase in clinical isolates of Enterobacter spp., a strain was found carrying the plasmid-mediated AmpC β-lactamase ACT-1 gene on its chromosome. The strain was identified as Enterobacter hormaechei using phylogenetic analysis of 16S rRNA and hsp60 genes. In addition, the species was confirmed by DNA-DNA hybridization. The genetic environment of the bla(ACT-1) gene was characterized, including the ampR and ampG genes, using a two-step PCR. The amino acid sequences of AmpR at serine 35, arginine 86, glycine 102, aspartic acid 135 and tyrosine 264 were conserved. Measurement of the transcription level of the bla(ACT-1) gene using real-time quantitative PCR showed that it increased 1.98-fold following cefoxitin induction. These results suggest that the plasmid-mediated bla(ACT-1) gene originated from the chromosome of E. hormaechei.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/90963
DOI
10.1099/jmm.0.032573-0
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Laboratory Medicine
Yonsei Authors
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