Cited 0 times in

Neuronal Differentiation of a Human Induced Pluripotent Stem Cell Line (FS-1) Derived from Newborn Foreskin Fibroblasts

Authors
 Jihye Kwon ; Nayeon Lee ; Jihwan Song ; Aeri Kim ; Dong Ah Shin ; Seung-Hun Oh ; Dong Ryul Lee ; Jeong Tae Do ; Hey Jin Lee ; Iksoo Jeon 
Citation
 International Journal of Stem Cells, Vol.5(2) : 140~145, 2012 
Journal Title
 International Journal of Stem Cells 
ISSN
 2005-3606 
Issue Date
2012
Abstract
Isolation of induced pluripotent stem cells (iPSCs) from fully differentiated somatic cells has revolutionized existing concepts of cell differentiation and stem cells. Importantly, iPSCs generated from somatic cells of patients can be used to model different types of human diseases. They may also serve as autologous cell sources that can be used in transplantation therapy. In this study, we investigated the neuronal properties of an iPSC line that is derived from human neonatal foreskin fibroblasts (FS-1). We initially examined the morphology and marker expression of FS-1 cells at undifferentiated stage. We then spontaneously differentiated FS-1 cells in suspension culture and examined the expression of markers representing three germ layers. We finally differentiated FS-1 cells into neuronal lineages by co-culturing them with PA6 stromal cells, and found that, under the conditions we used, they have a tendency to differentiate into more forebrain-type neurons, suggesting that FS-1 iPSC-derived neural cells will be useful to be used in cell therapy of stroke or Huntington’s disease, among others. Taken together, FS-1 cells derived from human neonatal fibroblasts exhibit very similar properties with human ES cells, and can provide useful sources for cell therapy and various other applications.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/90548
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Neurosurgery
Yonsei Authors
사서에게 알리기
  feedback
Files in This Item:
T201205669.pdfDownload
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse