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The novel function of Oct3/4 in mouse tooth development.

Authors
 Eizo Nakagawa ; Li Zhang ; Han-Sung Jung ; Hayato Ohshima ; Sung-Won Cho ; Jeong-Oh Shin ; Eun-Jung Kim 
Citation
 Histochemistry and Cell Biology, Vol.137(3) : 367~376, 2012 
Journal Title
 Histochemistry and Cell Biology 
ISSN
 0948-6143 
Issue Date
2012
Abstract
Octamer-binding factor 3/4 (Oct3/4) is one of the key regulators maintaining the pluripotency and self-renewal in embryonic stem cells and is involved in the developmental events. However, the functional significance of Oct3/4 remains to be clarified during tooth morphogenesis. This study aimed to examine the functional role of Oct3/4 in mouse. During tooth morphogenesis (E11-E16.5), Oct3/4-positive cells, detected by nuclear immunoreaction, increased in number, and subsequently, their immunoreaction shifted from the nucleus to the cytoplasm at the stage of cell differentiation (E18.5). Quantitative real-time PCR clearly demonstrated the relationship between isoforms of Oct3/4 and the in vivo cellular localization of Oct3/4, suggesting that the Oct3/4 expressed in nucleus was Oct3/4A, whereas that expressed in the cytoplasm was Oct3/4B. RNAi knockdown of Oct3/4 induced apoptosis and arrested tooth morphogenesis. Our results suggest that (1) the increased number of Oct3/4-positive cells with nuclear immunoreaction correlate with active cell proliferation during tooth morphogenesis and (2) the shift of Oct3/4 from the nucleus to the cytoplasm plays a crucial role in cell differentiation.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/90370
DOI
10.1007/s00418-011-0895-y
Appears in Collections:
1. 연구논문 > 2. College of Dentistry > Dept. of Oral Biology
Yonsei Authors
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Link
 http://link.springer.com/article/10.1007%2Fs00418-011-0895-y
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