Cited 19 times in

Different characteristics of reactive oxygen species production by human keratinocyte cell line cells in response to allergens and irritants.

Title
Different characteristics of reactive oxygen species production by human keratinocyte cell line cells in response to allergens and irritants.
Authors
Dong Hyun Kim;Dashlkhumbe Byamba;Min-Geol Lee;Tae-Gyun Kim;Wen H. Wu
Issue Date
2012
Journal Title
Experimental Dermatology
ISSN
0906-6705
Citation
Experimental Dermatology, Vol.21(2) : 99 ~103, 2012
Abstract
Keratinocytes mount immune responses through the secretion of a variety of inflammatory cytokines, soluble proteins and reactive oxygen species (ROS). However, the role of ROS in keratinocytes in response to allergens and irritants has not yet been elucidated. In this study, we investigated the (i) ROS production; (ii) potential sites of ROS production; (iii) expression of cell surface molecules; (iv) secretion of cytokines; and (v) ROS-dependent protein carbonylation in chemical-treated human keratinocyte cell line (HaCaT) cells. Treatment of HaCaT cells with 2,4-dinitrochlorobenzene (DNCB) and benzalkonium chloride (BKC) increased ROS levels in a time- and dose-dependent manner, as determined with dichlorodihydrofluorescein diacetate (CM-H(2) DCFDA), without reducing cell viability. Potential sources of ROS production were evaluated with pretreatment of diphenylene iodonium (DPI), an inhibitor of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase; rotenone, an inhibitor of the mitochondrial electron transport chain complex or allopurinol, a xanthine oxidase inhibitor. The DNCB-induced ROS was related to both NADPH oxidase and mitochondrial electron transport chain complex. Conversely, BKC-induced ROS was related to NADPH oxidase only. Western blotting using an anti-DNP antibody revealed ROS-dependent protein carbonylation in response to DNCB but not BKC. Both DNCB and BKC increased the secretion of IL-1α from HaCaT cells; however, ROS production as well as other changes, except DNCB-induced secretion of IL-1α, was not inhibited by antioxidants. Although the role of ROS in keratinocytes in response to chemicals was inconclusive, our results suggest that the characteristics of ROS produced by keratinocytes in response to chemicals might differ.
URI
http://onlinelibrary.wiley.com/doi/10.1111/j.1600-0625.2011.01399.x/abstract

http://ir.ymlib.yonsei.ac.kr/handle/22282913/90272
DOI
10.1111/j.1600-0625.2011.01399.x
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Dermatology
Yonsei Authors
사서에게 알리기
  feedback
Files in This Item:
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse