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Role of heat shock protein 47 in transdifferentiation of human tenon's fibroblasts to myofibroblasts.

Title
 Role of heat shock protein 47 in transdifferentiation of human tenon's fibroblasts to myofibroblasts.
Authors
 Samin Hong; Kyoungsoo Park; Gong Je Seong; Jong Bok Lee; Sueng-Han Han; Jin Hyoung Kim
Issue Date
2012
Journal Title
 BMC Ophthalmology
ISSN
 1471-2415
Citation
 BMC Ophthalmology, Vol.12(null) : 49, 2012
Abstract
BACKGROUND: Heat shock protein 47 (Hsp47) is a well-known molecular chaperone in collagen synthesis and maturation. The aim of this study is to investigate its putative role in the transdifferentiation of Tenon's fibroblasts to myofibroblasts. METHODS: Primary cultured human Tenon's fibroblasts were exposed to transforming growth factor-β1 (TGF-β1) for up to 48 hours. The mRNA levels of Hsp47 and α smooth muscle actin (αSMA) were determined by quantitative real time RT-PCR. After delivery of small interfering RNA (siRNA) molecules targeting Hsp47 into the cells, the expression of Hsp47 and αSMA proteins was determined by western immunoblotting. RESULTS: TGF-β1 increased the mRNA expressions of both Hsp47 and αSMA in human Tenon's fibroblasts, as determined by quantitative real time RT-PCR. However, it induced the protein expression of only αSMA but not Hsp47, as determined by western immunoblots. When siRNAs specific for Hsp47 were introduced into those cells, the TGF-β1-induced expression of αSMA was significantly attenuated on western immunoblots; after 48 hours of exposure to TGF-β1, the relative densities of immunobands were 11.58 for the TGF-β1 only group and 2.75 for the siRNA treatment group, compared with the no treatment control group (p < 0.001). CONCLUSIONS: Our data suggest that Hsp47 may be related to the TGF-β1-induced transdifferentiation of human Tenon's fibroblasts to myofibroblasts.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/89795
DOI
10.1186/1471-2415-12-49
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Pharmacology
1. 연구논문 > 1. College of Medicine > Dept. of Ophthalmology
Yonsei Authors
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