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Mussel-inspired immobilization of vascular endothelial growth factor (VEGF) for enhanced endothelialization of vascular grafts

Authors
 Young Min Shin  ;  Yu Bin Lee  ;  Seok Joo Kim  ;  Jae Kyeong Kang  ;  Jong-Chul Park  ;  Wonhee Jang  ;  Heungsoo Shin 
Citation
 BIOMACROMOLECULES, Vol.13(7) : 2020-2028, 2012 
Journal Title
BIOMACROMOLECULES
ISSN
 1525-7797 
Issue Date
2012
MeSH
Animals ; Bivalvia ; Blood Vessel Prosthesis* ; Cell Adhesion ; Cell Movement ; Cell Proliferation ; Cells, Cultured ; Human Umbilical Vein Endothelial Cells/physiology ; Humans ; Immobilized Proteins/chemistry* ; Indoles/chemistry ; Microscopy, Atomic Force ; Microscopy, Electron, Scanning ; Photoelectron Spectroscopy ; Platelet Endothelial Cell Adhesion Molecule-1/metabolism ; Polyesters/chemistry ; Polymers/chemistry ; Surface Properties ; Vascular Endothelial Growth Factor A/chemistry* ; Wettability
Keywords
Animals ; Bivalvia ; Blood Vessel Prosthesis* ; Cell Adhesion ; Cell Movement ; Cell Proliferation ; Cells, Cultured ; Human Umbilical Vein Endothelial Cells/physiology ; Humans ; Immobilized Proteins/chemistry* ; Indoles/chemistry ; Microscopy, Atomic Force ; Microscopy, Electron, Scanning ; Photoelectron Spectroscopy ; Platelet Endothelial Cell Adhesion Molecule-1/metabolism ; Polyesters/chemistry ; Polymers/chemistry ; Surface Properties ; Vascular Endothelial Growth Factor A/chemistry* ; Wettability
Abstract
Most polymeric vascular prosthetic materials have low patency rate for replacement of small diameter vessels (<5 mm), mainly due to failure to generate healthy endothelium. In this study, we present polydopamine-mediated immobilization of growth factors on the surface of polymeric materials as a versatile tool to modify surface characteristics of vascular grafts potentially for accelerated endothelialization. Polydopamine was deposited on the surface of biocompatible poly(L-lactide-co-ε-caprolactone) (PLCL) elastomer, on which vascular endothelial growth factor (VEGF) was subsequently immobilized by simple dipping. Surface characteristics and composition were investigated by using scanning electron microscopy, atomic force microscopy, and X-ray photoelectron spectroscopy. Immobilization of VEGF on the polydopamine-deposited PLCL films was effective (19.8 ± 0.4 and 197.4 ± 19.7 ng/cm(2) for DPv20 and DPv200 films, respectively), and biotin-mediated labeling of immobilized VEGF revealed that the fluorescence intensity increased as a function of the concentration of VEGF solution. The effect of VEGF on adhesion of HUVECs was marginal, which may have been masked by polydopamine layer that also enhanced cell adhesion. However, VEGF-immobilized substrate significantly enhanced proliferation of HUVECs for over 7 days of in vitro culture and also improved their migration. In addition, immobilized VEGF supported robust cell to cell interactions with strong expression of CD 31 marker. The same process was effective for immobilization of basic fibroblast growth factor, demonstrating the robustness of polydopamine layer for secondary ligation of growth factors as a simple and novel surface modification strategy for vascular graft materials.
Full Text
http://pubs.acs.org/doi/abs/10.1021/bm300194b
DOI
22617001
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
Kang, Jae Kyeong(강재경)
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/89722
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