Cited 3 times in

Activation of G Proteins by Aluminum Fluoride Enhances RANKLMediated Osteoclastogenesis

Title
Activation of G Proteins by Aluminum Fluoride Enhances RANKLMediated Osteoclastogenesis
Authors
Boryung Park;Yu-Mi Yang;Dong Min Shin;Min Seuk Kim;Byung-Jai Choi
Issue Date
2013
Journal Title
Korean Journal of Physiology & Pharmacology
ISSN
1226-4512
Citation
Korean Journal of Physiology & Pharmacology, Vol.17(5) : 427~433, 2013
Abstract
Receptor activator of NF-κB ligand (RANKL)-induced osteoclastogenesis is accompanied by intracellular Ca2+ mobilization in a form of oscillations, which plays essential roles by activating sequentially Ca2+/calmodulin-dependent protein kinase, calcineurin and NFATc1, necessary in the osteoclast differentiation. However, it is not known whether Ca2+ mobilization which is evoked in RANKL-independent way induces to differentiate into osteoclasts. In present study, we investigated Ca2+ mobilization induced by aluminum fluoride (AlF4-), a G-protein activator, with or without RANKL and the effects of AlF4- on the osteoclastogenesis in primary cultured mouse bone marrow-derived macrophages (BMMs). We show here that AlF4- induces intracellular Ca2+ concentration ([Ca2+]i) oscillations, which is dependent on extracellular Ca2+ influx. Notably, co-stimulation of AlF4- with RANKL resulted in enhanced NFATc1 expression and formation of tartrate-resistant acid phosphatase (TRAP) positive multinucleated cells. Additionally, we confirmed that mitogen-activated protein kinase (MAPK) is also activated by AlF4-. Taken together, these results demonstrate that G-protein would be a novel modulator responsible for [Ca2+]i oscillations and MAPK activation which lead to enhancement of RANKL-mediated osteoclastogenesis.
URI

http://ir.ymlib.yonsei.ac.kr/handle/22282913/88613
DOI
10.4196/kjpp.2013.17.5.427
Appears in Collections:
1. 연구논문 > 5. Research Institutes > 생체방어연구센터
1. 연구논문 > 2. College of Dentistry > Dept. of Oral Biology
1. 연구논문 > 2. College of Dentistry > Dept. of Pediatric Dentistry
Yonsei Authors
사서에게 알리기
  feedback
Files in This Item:
T201304309.pdfDownload
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse