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The Genome Sequence of ‘Mycobacterium massiliense’ Strain CIP 108297 Suggests the Independent Taxonomic Status of the Mycobacterium abscessus Complex at the Subspecies Level

Authors
 Yong-Joon Cho  ;  Hana Yi  ;  Jongsik Chun  ;  Sang-Nae Cho  ;  Charles L. Daley  ;  Won-Jung Koh  ;  Sung Jae Shin 
Citation
 PLOS ONE, Vol.8(11) : e81560, 2013 
Journal Title
PLOS ONE
Issue Date
2013
MeSH
Genome, Bacterial/genetics ; Genomics* ; Nontuberculous Mycobacteria/classification* ; Nontuberculous Mycobacteria/genetics* ; Phylogeny* ; Sequence Homology, Nucleic Acid
Keywords
Genome, Bacterial/genetics ; Genomics* ; Nontuberculous Mycobacteria/classification* ; Nontuberculous Mycobacteria/genetics* ; Phylogeny* ; Sequence Homology, Nucleic Acid
Abstract
Members of the Mycobacterium abscessus complex are rapidly growing mycobacteria that are emerging as human pathogens. The M. abscessus complex was previously composed of three species, namely M. abscessus sensu stricto, ‘M. massiliense’, and ‘M. bolletii’. In 2011, ‘M. massiliense’ and ‘M. bolletii’ were united and reclassified as a single subspecies within M. abscessus: M. abscessus subsp. bolletii. However, the placement of ‘M. massiliense’ within the boundary of M. abscessus subsp. bolletii remains highly controversial with regard to clinical aspects. In this study, we revisited the taxonomic status of members of the M. abscessus complex based on comparative analysis of the whole-genome sequences of 53 strains. The genome sequence of the previous type strain of ‘Mycobacterium massiliense’ (CIP 108297) was determined using next-generation sequencing. The genome tree based on average nucleotide identity (ANI) values supported the differentiation of ‘M. bolletii’ and ‘M. massiliense’ at the subspecies level. The genome tree also clearly illustrated that ‘M. bolletii’ and ‘M. massiliense’ form a distinct phylogenetic clade within the radiation of the M. abscessus complex. The genomic distances observed in this study suggest that the current M. abscessus subsp. bolletii taxon should be divided into two subspecies, M. abscessus subsp. massiliense subsp. nov. and M. abscessus subsp. bolletii, to correspondingly accommodate the previously known ‘M. massiliense’ and ‘M. bolletii’ strains.
Files in This Item:
T201304114.pdf Download
DOI
10.1371/journal.pone.0081560
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Shin, Sung Jae(신성재) ORCID logo https://orcid.org/0000-0003-0854-4582
Cho, Sang Nae(조상래)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/88556
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