Cited 0 times in

Galactosylated manganese ferrite nanoparticles for targeted MR imaging of asialoglycoprotein receptor

Authors
 Seung-Hyun Yang ; Dan Heo ; Sahng Wook Park ; Jaemoon Yang ; Yong-Min Huh ; Jin-Suck Suh ; Seungjoo Haam ; Young Han Lee ; Eun-Kyung Lim ; Eunjung Kim ; Eugene Lee 
Citation
 Nanotechnology, Vol.24(47) : 475103, 2013 
Journal Title
 Nanotechnology 
ISSN
 0957-4484 
Issue Date
2013
Abstract
Cancer cells can express specific biomarkers, such as cell membrane proteins and signaling factors. Thus, finding biomarkers and delivering diagnostic agents are important in the diagnosis of cancer. In this study, we investigated a biomarker imaging agent for the diagnosis of hepatic cancers. The asialoglycoprotein receptor (ASGPr) was selected as a biomarker for hepatoma cells and the ASGPr-targetable imaging agent bearing a galactosyl group was prepared using manganese ferrite nanoparticles (MFNP) and galactosylgluconic acid. The utility of the ASGPr-targetable imaging agent, galactosylated MFNP (G-MFNP) was assessed by several methods in ASGPr-expressing HepG2 cells as target cells and ASGPr-deficient MCF7 cells. Physical and chemical properties of G-MFNP were examined using Fourier-transform infrared spectroscopy, dynamic light scattering, zeta potential analysis, and transmission electron microscopy. No significant cytotoxicity was observed in either cell line. Targeting ability was assessed using flow cytometry, magnetic resonance imaging, inductively coupled plasma atomic emission spectroscopy, absorbance analysis, dark-field microscopy, Prussian blue staining, and transmission electron microscopy. We demonstrated that G-MFNP target successfully and bind to ASGPr-expressing HepG2 cells specifically. We suggest that these results will be useful in strategies for cancer diagnoses based on magnetic resonance imaging.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/88526
DOI
10.1088/0957-4484/24/47/475103
Appears in Collections:
1. 연구논문 > 5. Research Institutes > Institute for Medical Convergence
1. 연구논문 > 1. College of Medicine > Yonsei Biomedical Research Center
1. 연구논문 > 1. College of Medicine > Dept. of Radiology
1. 연구논문 > 5. Research Institutes > Yonsei Integrative Research Institute for Cerebral & Cardiovascular Disease
1. 연구논문 > 1. College of Medicine > Dept. of Biochemistry & Molecular Biology
Yonsei Authors
사서에게 알리기
  feedback
Link
 http://iopscience.iop.org/0957-4484/24/47/475103/
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse