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면역억제제에 의한 신장독성 연구에서 조직 미세배열분석 사용의 타탕성 검토

Title
면역억제제에 의한 신장독성 연구에서 조직 미세배열분석 사용의 타탕성 검토
Other Titles
Validation of Tissue Microarrays for the Study of Immunosuppressive Agent-induced Nephrotoxicity
Authors
임범진;김지홍;정현주
Issue Date
2013
Journal Title
Journal of the Korean Society for Transplantation (대한이식학회지)
ISSN
1598-1711
Citation
Journal of the Korean Society for Transplantation (대한이식학회지), Vol.27(3) : 114~120, 2013
Abstract
Background Tissue microarray analysis (TMA) is a high-throughput method for histologic evaluation, immunohistochemistry, and in situ hybridization using paraffin embedded tissue. Despite its high efficiency as an experimental tool, TMA is limited because it only contains a very small tissue fragment from each case. Therefore, the purpose of this study was to evaluate the validity of TMA in a study of nephrotoxicity caused by immunosuppressants. Methods Male Sprague-Dawley rats were treated with vehicle (n=16), cyclosporine (n=23), and cyclosporine plus losartan (n=13) for a maximum of 7 weeks. After animal sacrifice, renal tissues were embedded in paraffin and processed into slides for microscopic examination using conventional methods and the TMA technique. Acute tubular injury, vascular hyaline change, and interstitial fibrosis were scored in both conventional and TMA slides. The number of interstitial macrophages was counted after ED-1 immunohistochemistry and the results also compared between conventional and TMA slides. Results The degree of acute tubular injury and interstitial fibrosis showed a significant agreement between conventional and TMA methods (κ value, 0.79 and 1.00, respectively). The number of interstitial macrophages counted in conventional and TMA slides showed a significant correlation as well (r=0.934, P<0.001). However, the degree of vascular hyaline changes showed less agreement between conventional and TMA methods (κ value, 0.40). Conclusions TMA is a useful and reliable method for the study of nephrotoxicity induced by immunosuppressive agents. TMA also reflects the findings of conventional methods, especially for acute and chronic tubular and interstitial changes.
URI

http://ir.ymlib.yonsei.ac.kr/handle/22282913/88361
DOI
10.4285/jkstn.2013.27.3.114
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Pathology
1. 연구논문 > 1. College of Medicine > Dept. of Pediatrics
Yonsei Authors
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