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Allergenicity of recombinant profilins from Japanese hop, Humulus japonicus

Authors
 KY Jeong  ;  IS Han  ;  SY Choi  ;  JH Lee  ;  JS Lee  ;  CS Hong  ;  JW Park 
Citation
 JOURNAL OF INVESTIGATIONAL ALLERGOLOGY AND CLINICAL IMMUNOLOGY, Vol.23(5) : 345-350, 2013 
Journal Title
JOURNAL OF INVESTIGATIONAL ALLERGOLOGY AND CLINICAL IMMUNOLOGY
ISSN
 1018-9068 
Issue Date
2013
MeSH
Adult ; Aged ; Allergens/immunology* ; Amino Acid Sequence ; Female ; Humans ; Humulus/immunology* ; Immunoglobulin E/blood ; Male ; Middle Aged ; Molecular Sequence Data ; Profilins/chemistry ; Profilins/immunology* ; Recombinant Proteins/immunology ; Recombinant Proteins/isolation & purification
Keywords
Allergen. Humulus japonicus. Pollen allergens. Profilin. Recombinant allergens. Recombinant profilin
Abstract
BACKGROUND AND OBJECTIVE:
Pollen from Japanese hop, Humulusjaponicus, is a major cause of pollinosis in Korea. Profilin (15 kDa) from Humulus scandens has been associated with strong allergenicity in allergic Chinese patients. Profilin has also been detected in pollen extract from Korean Japanese hop by proteomic analysis and immunoglobulin (Ig) E immunoblotting. However, the allergenicity of allergens isolated from Japanese hop has not been investigated in Korean individuals. This study was undertaken to produce recombinant profilin from Japanese hop and evaluate its allergenicity.
METHODS:
Complementary DNA sequences encoding 2 isoallergens were cloned by reverse transcription polymerase chain reaction and their recombinant proteins expressed in Escherichia coli. The IgE-binding reactivities of the recombinant allergens were assessed by enzyme-linked immunosorbent assay.
RESULTS:
The deduced amino acid sequences of the H. japonicus profilins were 68.7% to 80.2% homologous with profilins from mugwort (Art v 4), ragweed (Amb a 14), and birch (Bet v 2). Two isoallergens of profilin from H. japonicus were 78.2% identical. Notably, the cDNA sequences of these 2 isoallergens were 98.5% (AY268422) and 98.7% (AY268424) identical to those of H. scandens. Serum samples from Japanese hop-sensitized individuals showed 12.9% IgE reactivity to both of the recombinant profilin isoallergens from H. japonicus, indicating that profilin may not be an allergenically dominant component of Japanese hop pollen. The recombinant profilins showed only 0% to 9.3% inhibition of the crude extract.
CONCLUSIONS:
Two isoallergens of profilin that are highly conserved with those of mugwort, ragweed, and birch were identified in H. japonicus. Profilins from Japanese hop pollen may play a minor role in the pathogenesis of pollinosis in Koreans.
Files in This Item:
T201303703.pdf Download
DOI
24260980
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Park, Jung Won(박중원) ORCID logo https://orcid.org/0000-0003-0249-8749
Lee, Jae Hyun(이재현) ORCID logo https://orcid.org/0000-0002-0760-0071
Jeong, Kyoung Yong(정경용) ORCID logo https://orcid.org/0000-0001-9887-1426
Choi, Soo Young(최수영)
Han, In Soo(한인수)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/88307
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