Cited 25 times in

Sensitive and specific assays for routine serological diagnosis of epidermolysis bullosa acquisita

Title
 Sensitive and specific assays for routine serological diagnosis of epidermolysis bullosa acquisita 
Authors
 Lars Komorowski ; Ralf Müller ; Enno Schmidt ; Winfried Stöcker ; Detlef Zillikens ; Ralf J. Ludwig ; Richard Groves ; Soo-Chan Kim ; Takashi Hashimoto ; Marcel F. Jonkman ; Andreas Recke ; Christian Probst ; Artem Vorobyev 
Issue Date
2013
Journal Title
 Journal of the American Academy of Dermatology 
ISSN
 0190-9622 
Citation
 Journal of the American Academy of Dermatology, Vol.68(3) : e89~e95, 2013 
Abstract
BACKGROUND: Epidermolysis bullosa acquisita (EBA) is a severe autoimmune subepidermal blistering disease characterized by autoantibodies against the N-terminal collagenous domain (NC1) of type VII collagen (Col VII). OBJECTIVE: Development of reliable assays for the detection of anti-Col VII-NC1 antibodies. METHODS: NC1 was expressed in human HEK293 cells and used as target antigen in an enzyme-linked immunosorbent assay (ELISA) and in an immunofluorescence assay (IFA). These two assays were probed in a large cohort of patients with EBA (n = 73), bullous pemphigoid (BP, n = 72), anti-p200 pemphigoid (n = 24), anti-laminin 332 mucous membrane pemphigoid (MMP, n = 15), pemphigus vulgaris (PV, n = 24), and healthy control subjects (n = 254). RESULTS: The cut-off for the ELISA was optimized for accuracy by receiver-operating characteristics (area under the curve [AUC] = 0.9952). IgG reactivity against NC1 was detected in 69 of 73 EBA (94.5%) and 5 control sera (2 healthy controls and 3 BP patients), resulting in a specificity of 98.7%. The IFA showed a sensitivity of 91.8% and specificity of 99.8%. Reproducibility of the ELISA was demonstrated by an intra-class correlation coefficient of 0.97. IgG subclass analyses by ELISA revealed IgG1, IgG2, IgG3, and IgG4 anti-NC1 reactivity in 83.6%, 85.3%, 37.7%, and 83.6% of EBA sera, respectively. LIMITATIONS: The novel assays were not evaluated prospectively and their use in monitoring serum levels during the disease course was not tested. CONCLUSION: The two assays are highly specific and sensitive to diagnose EBA. Their diagnostic competence was demonstrated in a large cohort of well-characterized EBA sera.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/88175
DOI
10.1016/j.jaad.2011.12.032
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Dermatology
Yonsei Authors
사서에게 알리기
  feedback
Link
 http://www.sciencedirect.com/science/article/pii/S0190962212000102
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse