Cited 26 times in

Sensitive and specific assays for routine serological diagnosis of epidermolysis bullosa acquisita

Authors
 Lars Komorowski ; Ralf Müller ; Enno Schmidt ; Winfried Stöcker ; Detlef Zillikens ; Ralf J. Ludwig ; Richard Groves ; Soo-Chan Kim ; Takashi Hashimoto ; Marcel F. Jonkman ; Andreas Recke ; Christian Probst ; Artem Vorobyev 
Citation
 Journal of the American Academy of Dermatology, Vol.68(3) : e89~e95, 2013 
Journal Title
 Journal of the American Academy of Dermatology 
ISSN
 0190-9622 
Issue Date
2013
Abstract
BACKGROUND: Epidermolysis bullosa acquisita (EBA) is a severe autoimmune subepidermal blistering disease characterized by autoantibodies against the N-terminal collagenous domain (NC1) of type VII collagen (Col VII). OBJECTIVE: Development of reliable assays for the detection of anti-Col VII-NC1 antibodies. METHODS: NC1 was expressed in human HEK293 cells and used as target antigen in an enzyme-linked immunosorbent assay (ELISA) and in an immunofluorescence assay (IFA). These two assays were probed in a large cohort of patients with EBA (n = 73), bullous pemphigoid (BP, n = 72), anti-p200 pemphigoid (n = 24), anti-laminin 332 mucous membrane pemphigoid (MMP, n = 15), pemphigus vulgaris (PV, n = 24), and healthy control subjects (n = 254). RESULTS: The cut-off for the ELISA was optimized for accuracy by receiver-operating characteristics (area under the curve [AUC] = 0.9952). IgG reactivity against NC1 was detected in 69 of 73 EBA (94.5%) and 5 control sera (2 healthy controls and 3 BP patients), resulting in a specificity of 98.7%. The IFA showed a sensitivity of 91.8% and specificity of 99.8%. Reproducibility of the ELISA was demonstrated by an intra-class correlation coefficient of 0.97. IgG subclass analyses by ELISA revealed IgG1, IgG2, IgG3, and IgG4 anti-NC1 reactivity in 83.6%, 85.3%, 37.7%, and 83.6% of EBA sera, respectively. LIMITATIONS: The novel assays were not evaluated prospectively and their use in monitoring serum levels during the disease course was not tested. CONCLUSION: The two assays are highly specific and sensitive to diagnose EBA. Their diagnostic competence was demonstrated in a large cohort of well-characterized EBA sera.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/88175
DOI
10.1016/j.jaad.2011.12.032
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Dermatology
Yonsei Authors
사서에게 알리기
  feedback
Link
 http://www.sciencedirect.com/science/article/pii/S0190962212000102
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse