PACS is a medical system for digital medical images, and PACS expand to web-based service using public network, DICOM files should be protected from the man-in-the-middle attack because they have personal medical record. To solve the problem, we designed flexible secure transmission system using IPSec and adopted to a web-based three-dimensional medical image system. And next, we performed the performance evaluation changing integrity and encryption algorithm using DICOM volume dataset. At that time, combinations of the algorithm was ‘DES-MD5’, ‘DES-SHA1’, ‘3DES-MD5’, and ‘3DES-SHA1, and the experiment was performed on our test-bed. In experimental result, the overall performance was affected by encryption algorithms than integrity algorithms, DES was approximately 50% of throughput degradation and 3DES was about to 65% of throughput degradation. Also when DICOM volume dataset was transmitted using secure transmission system, the network performance degradation had shown because of increased packet overhead. As a result, server and network performance degradation occurs for secure transmission system by ensuring the secure exchange of messages. Thus, if the secure transmission system adopted to the medical images that should be protected, it could solve server performance gradation and compose secure web PACS.