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Evaluation of the Bioefficacy of a Stabilized Form of Human Growth Hormone (SP-hGH)

Authors
 S.-B. Lee  ;  H. Park  ;  J. Koh  ;  H. Lee  ;  J. Kim 
Citation
 HORMONE AND METABOLIC RESEARCH, Vol.45(10) : 722-727, 2013 
Journal Title
HORMONE AND METABOLIC RESEARCH
ISSN
 0018-5043 
Issue Date
2013
MeSH
Animals ; Blood Glucose/drug effects ; Body Weight/drug effects ; Cell Proliferation/drug effects ; Drug Evaluation, Preclinical ; Drug Stability ; Female ; Human Growth Hormone/administration & dosage ; Human Growth Hormone/chemistry* ; Human Growth Hormone/pharmacology* ; Humans ; MCF-7 Cells ; Mice ; Mice, Inbred ICR ; Protein Stability ; Recombinant Proteins/administration & dosage ; Recombinant Proteins/chemistry ; Recombinant Proteins/pharmacology ; Treatment Outcome
Keywords
protein aggregation ; protein stability ; stabilizing peptide ; human growth hormone
Abstract
Protein aggregation is a major obstacle in maintaining the stability of therapeutic proteins. In previous studies, fusion between a stabilizing peptide (SP) and human growth hormone (hGH) resulted in improved solubility and stability compared with hGH alone, although the bioactivity of the protein was not confirmed in vivo. In this study, we evaluated the bioefficacy of hGH and SP-hGH in vivo using a mouse model. Subcutaneous injections of 30 μg of hGH or SP-hGH were administered to 8-month-old female mice, twice a week for 14 weeks. Neither hGH nor SP-hGH significantly affected body weight or blood glucose levels compared with control mice. Interestingly, abdominal fat was significantly reduced in SP-hGH-treated mice compared with hGH-treated mice. While total cholesterol, HDL, and LDL levels were slightly higher in both groups, TG levels were significantly reduced in both SP-hGH and hGH-treated mice compared with control mice. IGF-1 levels in the liver were increased in both the SP-hGH and hGH groups, thereby inducing liver cell proliferation. These results suggest that SP fusion with hGH attained similar or improved bioefficacy compared with hGH alone.
Full Text
https://www.thieme-connect.com/DOI/DOI?10.1055/s-0033-1345126
DOI
10.1055/s-0033-1345126
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Jong Sun(김종선) ORCID logo https://orcid.org/0000-0002-3149-669X
Park, Hyun Ju(박현주)
Lee, Saet Byul(이샛별)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/88066
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