약제내성 Mycobacterium tuberculosis의 rpoB 유전자 분석과 클로닝 발현
Analysis and Expression of Cloning of rpoB Gene of Drug-Resistant Mycobacterium tuberculosis
최은경 ; 권태동 ; 홍성갑 ; 조해선 ; 배선준
한국정보통신학회논문지, Vol.17(4) : 1005~1009, 2013
Using DNA sequencing method, we analyzed mutations of rpoB (RNA polymerase beta subunit) rifampin-resistant Mycobaterium tuberculosis strains which were identified by conventional test at Masan National Hospital and The Korean Institute of Tuberculosis. Though it has been reported different mutations of rpoB region of rifampin-resistant M. tuberculosis strains in the south of Korea, it is not confirmed whether these mutations of rpoB region actually express rifampin resistance through experiment. We confirmed experimentally these mutations of rpoB region of M. tuberculosis strains induced rifampin-resistance through ampified rpoB by polymerase chain reaction (PCR) and cloning of mutant rpoB into rifampin sensitive-M. tuberculosis strain.