Cited 7 times in

Environmental Enrichment Synergistically Improves Functional Recovery by Transplanted Adipose Stem Cells in Chronic Hypoxic-Ischemic Brain Injury

Title
 Environmental Enrichment Synergistically Improves Functional Recovery by Transplanted Adipose Stem Cells in Chronic Hypoxic-Ischemic Brain Injury 
Authors
 Seo, Jung Hwa ;  Kim, Hyongbum ;  Cho, Sung-Rae ;  Kim, Chul Hoon ;  Lee, Min-Young ;  Kim, Ji Yeon ;  Yu, Ji Hea ;  Im, Sang Hee ;  Kim, Hyun Ok ;  Kim, Dong Wook ;  Lee, Jong Eun ;  Park, Eun Sook 
Issue Date
2013
Journal Title
 Cell Transplantation 
ISSN
 0963-6897 
Citation
 Cell Transplantation, Vol.22(9) : 1553~1568, 2013 
Abstract
We investigated the effects of environmental enrichment (EE) on the function of transplanted adipose stem cells (ASCs) and the combined effect of EE and ASC transplantation on neurobehavioral function in an animal model of chronic hypoxic-ischemic (HI) brain injury. HI brain damage was induced in 7-day-old mice by unilateral carotid artery ligation and exposure to hypoxia (8% O2 for 90 min). At 6 weeks of age, the mice were randomly injected with either ASCs or PBS into the striatum and were randomly assigned to either EE or standard cages (SC), comprising ASC-EE (n=18), ASC-SC (n=19), PBS-EE (n=12), PBS-SC (n=17), and untreated controls (n=23). Rotarod, forelimb-use asymmetry, and grip strength tests were performed to evaluate neurobehavioral function. The fate of transplanted cells and the levels of endogenous neurogenesis, astrocyte activation, and paracrine factors were also measured. As a result, EE and ASC transplantation synergistically improved rotarod latency, forelimb-use asymmetry, and grip strength compared to those of the other groups. The number of engrafted ASCs and βIII-tubulin(+) neurons derived from the transplanted ASCs was significantly higher in mice in EE than those in SC. EE and ASC transplantation also synergistically increased BrdU(+)βIII-tubulin(+) neurons, GFAP(+) astrocytic density, and fibroblast growth factor 2 (FGF2) level but not the level of CS-56(+) glial scarring in the striatum. In conclusion, EE and ASC transplantation synergistically improved neurobehavioral functions. The underlying mechanisms of this synergism included enhanced repair processes such as higher engraftment of the transplanted ASCs, increased endogenous neurogenesis and astrocytic activation coupled with upregulation of FGF2.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/87655
DOI
10.3727/096368912X662390
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Anatomy
1. 연구논문 > 1. College of Medicine > Dept. of Rehabilitation Medicine
1. 연구논문 > 1. College of Medicine > Dept. of Laboratory Medicine
1. 연구논문 > 1. College of Medicine > Dept. of Pharmacology
1. 연구논문 > 1. College of Medicine > Dept. of Physiology
1. 연구논문 > 1. College of Medicine > Yonsei Biomedical Research Center
Yonsei Authors
사서에게 알리기
  feedback
Link
 http://www.ingentaconnect.com/content/cog/ct/2013/00000022/00000009/art00005?token=0055188bbe1c5a927b76504c4866462544495b2f784038597c6a332b25757d5c4f6d4e227a3ea38599370
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse