Cited 9 times in

Standardization of multilocus sequence typing scheme for Mycobacterium abscessus and Mycobacterium massiliense

Title
Standardization of multilocus sequence typing scheme for Mycobacterium abscessus and Mycobacterium massiliense
Authors
Song Yee Kim;Young Ae Kang;Seok Hoon Jeong;Joon Chang;Se Kyu Kim;Young Sam Kim;Moo Suk Park;Jae-Joon Yim;Il Kwon Bae
Issue Date
2013
Journal Title
Diagnostic Microbiology and Infectious Disease
ISSN
0732-8893
Citation
Diagnostic Microbiology and Infectious Disease, Vol.77(2) : 143~149, 2013
Abstract
This study aims to develop a multilocus sequence typing (MLST) scheme for Mycobacterium abscessus complex for the typing of stains within each species. A total of 89 clinical isolates of M. abscessus complex from 71 patients of 2 tertiary care hospitals in South Korea were included. Forty-two isolates were identified as M. abscessus, and 29, as Mycobacterium massiliense through sequencing of 8 housekeeping genes and rpoB. The MLST scheme identified 26 different sequence types(STs) and 13 different clonal complexes (CCs) in M. abscessus and 12 different STs and 6 different CCs in M. massiliense. The MLST data showed high concordance with the XbaI-macrorestriction patterns of pulsed-field gel electrophoresis in the duplicated isolates. Our MLST schemes could identify different strains of M. abscessus and M. massiliense, and the schemes also showed a reliable reproducibility. Therefore, our MLST schemes may be useful in studying the epidemiology of M. abscessus and M. massiliense infections.
URI
http://www.sciencedirect.com/science/article/pii/S0732889313003787

http://ir.ymlib.yonsei.ac.kr/handle/22282913/87654
DOI
10.1016/j.diagmicrobio.2013.06.023
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Laboratory Medicine
1. 연구논문 > 1. College of Medicine > Dept. of Internal Medicine
Yonsei Authors
사서에게 알리기
  feedback
Files in This Item:
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse