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Standardization of multilocus sequence typing scheme for Mycobacterium abscessus and Mycobacterium massiliense

Authors
 Song Yee Kim ; Young Ae Kang ; Seok Hoon Jeong ; Joon Chang ; Se Kyu Kim ; Young Sam Kim ; Moo Suk Park ; Jae-Joon Yim ; Il Kwon Bae 
Citation
 Diagnostic Microbiology and Infectious Disease, Vol.77(2) : 143~149, 2013 
Journal Title
 Diagnostic Microbiology and Infectious Disease 
ISSN
 0732-8893 
Issue Date
2013
Abstract
This study aims to develop a multilocus sequence typing (MLST) scheme for Mycobacterium abscessus complex for the typing of stains within each species. A total of 89 clinical isolates of M. abscessus complex from 71 patients of 2 tertiary care hospitals in South Korea were included. Forty-two isolates were identified as M. abscessus, and 29, as Mycobacterium massiliense through sequencing of 8 housekeeping genes and rpoB. The MLST scheme identified 26 different sequence types(STs) and 13 different clonal complexes (CCs) in M. abscessus and 12 different STs and 6 different CCs in M. massiliense. The MLST data showed high concordance with the XbaI-macrorestriction patterns of pulsed-field gel electrophoresis in the duplicated isolates. Our MLST schemes could identify different strains of M. abscessus and M. massiliense, and the schemes also showed a reliable reproducibility. Therefore, our MLST schemes may be useful in studying the epidemiology of M. abscessus and M. massiliense infections.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/87654
DOI
10.1016/j.diagmicrobio.2013.06.023
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Laboratory Medicine
1. 연구논문 > 1. College of Medicine > Dept. of Internal Medicine
Yonsei Authors
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Link
 http://www.sciencedirect.com/science/article/pii/S0732889313003787
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