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Identification of α-11 giardin as a flagellar and surface component of Giardia lamblia

Authors
 Juri Kim ; Hye Yeon Lee ; Soon-Jung Park ; Kyu-Ho Lee ; Tai-Soon Yong ; Mi-Ae Lee 
Citation
 Experimental Parasitology, Vol.135(2) : 227~233, 2013 
Journal Title
 Experimental Parasitology 
ISSN
 0014-4894 
Issue Date
2013
Abstract
Giardia lamblia is a protozoan pathogen with distinct cytoskeletal structures, including median bodies and eight flagella. In this study, we examined components comprising G. lamblia flagella. Crude flagellar extracts were prepared from G. lamblia trophozoites, and analyzed by two-dimensional (2-D) gel electrophoresis. The 19 protein spots were analyzed by MALDI-TOF mass spectrometry, identifying ten metabolic enzymes, six distinct giardins, Giardia trophozoite antigen 1, translational initiation factor eIF-4A, and an extracellular signal-regulated kinase 2. Among the identified proteins, we studied α-11 giardin which belongs to a group of cytoskeletal proteins specific to Giardia. Western blot analysis and real-time PCR indicated that expression of α-11 giardin is not significantly increased during encystation of G. lamblia. Immunofluorescence assays using anti-α-11 giardin antibodies revealed that α-11 giardin protein mainly localized to the plasma membranes and basal bodies of the anterior flagella of G. lamblia trophozoites, suggesting that α-11 giardin is a genuine component of the G. lamblia cytoskeleton.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/87573
DOI
10.1016/j.exppara.2013.07.010
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Environmental Medical Biology
Yonsei Authors
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Link
 http://www.sciencedirect.com/science/article/pii/S0014489413001963
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