Cited 6 times in

Conditional Deletion of Pten Leads to Defects in Nerve Innervation and Neuronal Survival in Inner Ear Development

Title
Conditional Deletion of Pten Leads to Defects in Nerve Innervation and Neuronal Survival in Inner Ear Development
Authors
Hyung Jin Kim;Hae-Mi Woo;Soo Kyung Koo;Hyun-Young Park;Mi-Hyun Park;Sang Back Choi;Jin Woo Kim;Jinwoong Bok;Jihee Ryu
Issue Date
2013
Journal Title
PLoS One
ISSN
1932-6203
Citation
PLoS One, Vol.8(2) : e55609, 2013
Abstract
All cellular phenomena and developmental events, including inner ear development, are modulated through harmonized signaling networks. Phosphatase and tensin homolog deleted on chromosome 10 (PTEN), a tumor suppressor, is a major signaling component involved in cross talk with key regulators of development; i.e., Wnt, Notch, and bone morphogenetic proteins. Although Pten function has been studied in various systems, its role in inner ear development is poorly understood. Here, we used inner ear-specific Pten conditional knockout mice and examined the characteristics of the inner ear. In a detailed analysis of the phenotype, reduced cochlear turning and widened epithelia were observed. Phalloidin staining of sensory epithelium revealed that hair cell patterns were disturbed; i.e., additional rows of hair cells were discovered. The neural abnormality revealed a reduction in and disorganization of nerve fibers, including apoptosis at the neural precursor stage. Pten deficiency induced increased phosphorylation of Akt at Ser473. The elevation of inhibitory glycogen synthase kinase 3β Ser9 phosphorylation (pGSK3β) was sustained until the neuronal differentiation stage at embryonic day 14.5, instead of pGSK3β downregulation. This is the first report on the influence of Pten/Akt/GSK3β signaling on the development of spiral ganglia. These results suggest that Pten is required for the maintenance of neuroblast number, neural precursors, and differentiation in the inner ear.
URI

http://ir.ymlib.yonsei.ac.kr/handle/22282913/87469
DOI
10.1371/journal.pone.0055609
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Anatomy
Yonsei Authors
사서에게 알리기
  feedback
Files in This Item:
T201302605.pdfDownload
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse