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Isolation of mesenchymal stem-like cells in meningioma specimens

Title
Isolation of mesenchymal stem-like cells in meningioma specimens
Authors
Hyo-Yeol Lim;Kyung Min Kim;Bo Kyung Kim;Jin-Kyoung Shim;Ji-Hyun Lee;Yong-Min Huh;Se-Hoon Kim;Eui-Hyun Kim;Eun-Kyung Park;Kyu-Won Shim;Jong Hee Chang;Dong-Seok Kim;Sun Ho Kim;Yong-Kil Hong;Su-Jae Lee;Seok-Gu Kang
Issue Date
2013
Journal Title
International Journal of Oncology
ISSN
1019-6439
Citation
International Journal of Oncology, Vol.43(4) : 1260~1268, 2013
Abstract
Cells resembling bone marrow mesenchymal stem cells (BM-MSCs) have been isolated from glioma specimens; however, little is known about the existence of mesenchymal stem-like cells (MSLCs) in meningioma. Here, we hypothesized that cells similar to BM-MSCs exist in meningioma specimens and sought to investigate whether these putative meningioma stroma MSLCs (MS-MSLCs) could be isolated. To this end, we cultured fresh meningioma specimens using the same protocols as used previously to isolate BM-MSC. Cultured cells were analyzed for surface markers associated with BM-MSCs by fluorescence-activated cell sorting (FACS) and candidate cells were exposed to mesenchymal differentiation conditions. Possible locations of MS-MSLCs were determined by immunohistochemical analysis of sections of meningioma specimens. Spindle-shaped and, adherent cells similar to BM-MSCs were isolated in 2 of 20 meningioma specimens. FACS analysis showed that the surface markers of MS-MSLCs were similar to those of BM-MSCs and the chosen cells demonstrated an ability to differentiate into osteogenic, adipogenic and chondrogenic cells. The tumorigenicity of MS-MSLCs was tested by injection of these cells into the brain of athymic nude mice; no tumors were subsequently discovered. Immunohistochemical analyses indicated that CD105+ cells were closely associated with endothelial cells and pericytes in meningioma specimens. Our results established for the first time that cells similar to BM-MSCs exist in meningioma specimens. These cells, termed MS-MSLCs, could be one component of the meningioma cellular microenvironment.
URI
http://www.spandidos-publications.com/ijo/43/4/1260

http://ir.ymlib.yonsei.ac.kr/handle/22282913/87259
DOI
10.3892/ijo.2013.2053
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Yonsei Biomedical Research Center
1. 연구논문 > 1. College of Medicine > Dept. of Neurosurgery
1. 연구논문 > 1. College of Medicine > Dept. of Pathology
1. 연구논문 > 1. College of Medicine > Dept. of Radiology
Yonsei Authors
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