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Pax6 Expressed in Osteocytes Inhibits Canonical Wnt Signaling

Authors
 Eun Jin Kim ; Ajita Jami ; Sung-Kil Lim ; Hong-Hee Kim ; Mi Jeong Lee ; Han-Sung Jung ; Min Jung Lee ; Jogeswar Gadi 
Citation
 Molecules and Cells, Vol.35(4) : 305~312, 2013 
Journal Title
 Molecules and Cells 
ISSN
 1016-8478 
Issue Date
2013
Abstract
The transcription factor Pax6, which belongs to the paired box-containing gene family, regulates developmental processes, especially in the eyes, central nervous tissues and craniofacial structures. However, the role of Pax6 in bone has never been studied exclusively. Here we report that Pax6 is expressed at both the mRNA and protein level in the calvaria and long bones of adult mice as well as osteocyte-like MLOY4 cells and suppresses the canonical Wnt signaling pathway. Moreover, the expression levels of Pax6 were much higher in the calvaria than the long bones, and Pax6 was also expressed at E16 to E18 in both the calvaria and long bones. Knockdown of Pax6 in MLOY4 cells did not affect cell proliferation or survival; however, the expression of Sost, an osteocyte marker gene, was significantly decreased. In addition, the overexpression of Pax6 suppressed the canonical Wnt signaling pathway by enhancing the expression of Sost. Furthermore, we also demonstrated that Pax6 binds to the Sost promoter and that stimulation of Sost transcription by Pax6 was dependent on a specific Pax6-binding sequence within the promoter. In conclusion, the results of the present study suggest that Pax6 is expressed in bone and may play an important role in osteocyte differentiation by controlling canonical Wnt signaling.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/87240
DOI
10.1007/s10059-013-2310-0
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Yonsei Biomedical Research Center
1. 연구논문 > 5. Research Institutes > Institute of Endocrinology
1. 연구논문 > 1. College of Medicine > Dept. of Life Science
1. 연구논문 > 2. College of Dentistry > Dept. of Oral Biology
1. 연구논문 > 1. College of Medicine > Dept. of Internal Medicine
Yonsei Authors
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