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Association of genetic variation in chitotriosidase with atopy in Korean children

Authors
 Kyung Won Kim ; Joonhee Park ; Kyu-Earn Kim ; Myung Hyun Sohn ; Min Goo Lee ; Won Il Heo ; Mi Na Kim ; Jung Yeon Hong ; Kyung Eun Lee ; Keun-Hwa Lee ; Jaechun Lee ; Hee Seon Lee ; Ji Hyun Lee 
Citation
 Annals of Allergy, Asthma & Immunology, Vol.110(6) : 444~449, 2013 
Journal Title
 Annals of Allergy, Asthma & Immunology 
ISSN
 1081-1206 
Issue Date
2013
Abstract
BACKGROUND: The atopic diseases, which are the most common chronic diseases of childhood, are complex genetic diseases that involve the contribution of multiple genetic factors to disease pathophysiology. Chitotriosidase is involved in innate immunity, but the association of chitotriosidase with allergic diseases remains unclear. OBJECTIVE: To examine the contribution of genetic variation of the chitotriosidase-encoding gene CHIT1 to atopic phenotypes in a Korean cohort of children. METHODS: We identified CHIT1 variations in a Korean population and conducted association analyses using 295 atopic and 242 nonatopic children. An independent replication study was performed using DNA samples from 148 atopic and 243 nonatopic children. All children were unrelated. We performed Western blot analysis in each genotype in vitro to see whether the CHIT1 A442G variation affects the final protein expression levels. RESULTS: In the case-control association analysis, atopy was significantly associated with a single A442G (rs1065761) polymorphism in CHIT1 (odds ratio = 1.32, P = .01). Children with the c.442G risk allele had significantly higher blood eosinophils (P = .001), total serum IgE (P = .007), and eosinophil cationic protein (P = .02) levels. The results of the replication stage analysis confirmed a significant association between the A442G polymorphism and childhood atopy. The joint analysis of the exploratory and replication studies displayed a stronger significant association. The relative protein expression levels of chitotriosidase were significantly higher in both cell lysate and media with the G transfection compared with the wild type. CONCLUSION: These results indicate that the nonsynonymous A442G polymorphism in CHIT1 is associated with risk of atopy.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/87225
DOI
10.1016/j.anai.2013.03.009
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Pediatrics
1. 연구논문 > 5. Research Institutes > 생체방어연구센터
1. 연구논문 > 1. College of Medicine > Dept. of Pharmacology
1. 연구논문 > 1. College of Medicine > Yonsei Biomedical Research Center
Yonsei Authors
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Link
 http://www.sciencedirect.com/science/article/pii/S1081120613002044
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