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A microfluidic array for quantitative analysis of human neural stem cell self-renewal and differentiation in three-dimensional hypoxic microenvironment

Authors
 Kisuk Yang ; Sewoon Han ; Seung-Woo Cho ; Seok Chung ; Kook In Park ; Jin Kim ; Eunkyung Ko ; Yoojin Shin 
Citation
 Biomaterials, Vol.34(28) : 6607~6614, 2013 
Journal Title
 Biomaterials 
ISSN
 0142-9612 
Issue Date
2013
Abstract
We report a microfluidic array for investigating and quantitatively analyzing human neural stem cell (hNSC) self-renewal and differentiation in an in vivo-like microenvironment. NSC niche conditions, including three-dimensional (3D) extracellular matrices and low oxygen tension, were effectively reconstituted in the microfluidic array in a combinatorial manner. The array device was fabricated to be detachable, rendering it compatible with quantitative real-time polymerase chain reaction for quantifying the effects of the biomimetic conditions on hNSC self-renewal and differentiation. We show that throughput of 3D cell culture and quantitative analysis can be increased. We also show that 3D hypoxic microenvironments maintain hNSC self-renewal capacity and direct neuronal commitment during hNSC differentiation.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/87097
DOI
10.1016/j.biomaterials.2013.05.067
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Pediatrics
Yonsei Authors
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Link
 http://www.sciencedirect.com/science/article/pii/S0142961213006595
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