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A Method for Electroporation to Study Gene Function in Mammary Gland Development

Authors
 김은정 ; 이종민 ; 정한성 
Citation
 Journal of Mammary Gland Biology and Neoplasia, Vol.18(2) : 233~237, 2013 
Journal Title
 Journal of Mammary Gland Biology and Neoplasia 
ISSN
 1083-3021 
Issue Date
2013
Abstract
To reveal the specific functions of various genes during embryonic development, the manipulation of genes using techniques such as electroporation is of fundamental importance for providing direct evidence concerning function or downstream activation of signaling networks. In vitro embryo culture and electroporation are useful techniques to introduce foreign genes, for developmental biology studies. Among the various mammalian culture techniques, Trowell culture is suitable for studies of embryonic mammary gland development because of its stability and ease of use in conjunction with electroporation technique application. The manipulation of gene expression using electroporation is a useful technique for the functional analysis of a particular gene. In this protocol, full steps for electroporation and in vitro embryo culture have been described for use in embryonic mammary gland development research.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/87003
DOI
10.1007/s10911-013-9292-6
Appears in Collections:
1. 연구논문 > 2. College of Dentistry > Dept. of Oral Biology
Yonsei Authors
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Link
 http://link.springer.com/article/10.1007%2Fs10911-013-9292-6
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