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Identification of Novel Allergenic Components from German Cockroach Fecal Extract by a Proteomic Approach

Authors
 Jeong K.Y. ; Kim C. ; Yong T.-S. ; Park J.-W. ; Han I.-S. ; Park J. 
Citation
 International Archives of Allergy and Immunology, Vol.161(4) : 315~324, 2013 
Journal Title
 International Archives of Allergy and Immunology 
ISSN
 1018-2438 
Issue Date
2013
Abstract
BACKGROUND: Cockroaches produce potent allergens, and cockroach feces are known to be especially rich in allergens. In this study, we analyze the allergenic components from cockroach feces and evaluate allergenicity of recombinant α-amylase identified from fecal extract. METHODS: IgE-reactive proteins from German cockroach fecal extract were analyzed by proteomic analysis and immunoblotting. Recombinant α-amylase was produced and its allergenicity was evaluated by ELISA. RESULTS: Analysis of German cockroach fecal extracts identified 12 IgE-reactive components. Most of these allergens were found to be digestive enzymes such as α-amylase, trypsin, chymotrypsin, metalloprotease, and midgut carboxypeptidase A, but the identity of 3 IgE-reactive proteins is still unknown. Glycinin-like proteins, which were likely derived from the cockroach diet, were also identified. German cockroach α-amylase shares the highest identity with pig α-amylase (55.8%), followed by mite group 4 allergens (Blo t 4, 50.4%; Der p 4, 49.8%; Eur m 4, 47.4%). In this study, recombinant α-amylase from German cockroach was expressed, and its allergenicity was examined by ELISA. Specific IgE against recombinant amylase was detected in 41.4% (12/29) of serum samples from German cockroach-sensitized subjects. Recombinant α-amylase was able to inhibit 55% of specific IgE to German cockroach whole-body extract. CONCLUSIONS: Amylase was found to be an important novel allergen in cockroach feces. It is hoped that recombinant α-amylase will be useful for further studies and clinical applications.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/86952
DOI
10.1159/000347034
Appears in Collections:
1. 연구논문 > 2. College of Dentistry > Dept. of Oral Biology
1. 연구논문 > 1. College of Medicine > Dept. of Environmental Medical Biology
1. 연구논문 > 1. College of Medicine > Dept. of Internal Medicine
1. 연구논문 > 1. College of Medicine > Yonsei Biomedical Research Center
1. 연구논문 > 5. Research Institutes > Institute of Allergy
Yonsei Authors
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Link
 http://www.karger.com/Article/FullText/347034
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