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Hyaluronic acid receptor-targetable imidazolized nanovectors for induction of gastric cancer cell death by RNA interference

Authors
 Eunjung Kim ; Jaemoon Yang ; Seungjoo Haam ; Yong-Min Huh ; Jin-Suck Suh ; Jae-Ho Cheong ; Taeyun Kwon ; Gyudo Lee ; Eun-Kyung Lim ; Yonghee An ; Hyun-Ouk Kim 
Citation
 Biomaterials, Vol.34(17) : 4327~4338, 2013 
Journal Title
 Biomaterials 
ISSN
 0142-9612 
Issue Date
2013
Abstract
We have developed a nanovector consisting of hyaluronic acid (HA) and poly-l-lysine-graft-imidazole (PLI)-based polyplexes containing Bcl-xL-specific shRNA-encoding plasmid DNA (HA/PLI/pDNA) for CD44 targeted gastric cancer therapy. The prepared ternary polyplexes have a negative surface charge of −24 mV and a size of approximately 100 nm at an N/P ratio of 5 with HA/PLI molar ratio of 0.03. Gel electrophoresis and cell viability experiments demonstrated that the ternary polyplexes showed high stability and no cytotoxicity due to the anchored HA molecules on the surface of PLI/pDNA binary polyplexes. Selective cancer cell death was achieved by CD44-mediated gene delivery and the internalized gene was effectively escaped from endosomes due to the buffering capacity of imidazole groups in an acidic environment. These nanovectors may be highly efficient gene delivery tools that allow the selective destruction of metastatic gastric cancer cells.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/86812
DOI
10.1016/j.biomaterials.2013.02.006
Appears in Collections:
1. 연구논문 > 5. Research Institutes > Institute for Medical Convergence
1. 연구논문 > 5. Research Institutes > Yonsei Integrative Research Institute for Cerebral & Cardiovascular Disease
1. 연구논문 > 1. College of Medicine > Dept. of Surgery
1. 연구논문 > 1. College of Medicine > Dept. of Radiology
Yonsei Authors
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Link
 http://www.sciencedirect.com/science/article/pii/S0142961213001750
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