No Detection of Simian Virus 40 in Malignant Mesothelioma in Korea
Minseob Eom ; Jamshid Abdul-Ghafar ; Soon-Hee Jung ; Myoung Ja Chung ; Yoo Duk Choi ; Hye Kyoung Yoon ; Chang Hun Lee ; Kyo Young Lee ; Seung Yeon Ha ; Wan Seop Kim ; Lucia Kim ; Eun Suk Ko ; Kun Young Kwon ; Soon Won Hong ; Joung Ho Han ; Sun-Mi Park
Korean Journal of Pathology (대한병리학회지), Vol.47(2) : 124~129, 2013
Korean Journal of Pathology (대한병리학회지)
Background: Simian virus 40 (SV40), a polyomavirus, was discovered as a contaminant of a human polio vaccine in the 1960s. It is known that malignant mesothelioma (MM) is associated with SV40, and that the virus works as a cofactor to the carcinogenetic effects of asbestos. However, the reports about the correlation between SV40 and MM have not been consistent. The purpose of this study is to identify SV40 in MM tissue in Korea through detection of SV40 protein and DNA. Methods: We analyzed 62 cases of available paraffin-blocks enrolled through the Korean Malignant Mesothelioma Surveillance System and performed immunohistochemistry for SV40 protein and real-time polymerase chain reaction (PCR) for SV40 DNA. Results: Of 62 total cases, 40 had disease involving the pleura (64.5%), and 29 (46.8%) were found to be of the epithelioid subtype. Immunostaining demonstrated that all examined tissues were negative for SV40 protein. Sufficient DNA was extracted for real-time PCR analysis from 36 cases. Quantitative PCR of these samples showed no increase in SV40 transcript compared to the negative controls. Conclusions: SV40 is not associated with the development of MM in Korea.