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Differential Immune Responses to Segniliparus rotundus and Segniliparus rugosus Infection and Analysis of Their Comparative Virulence Profiles

Title
 Differential Immune Responses to Segniliparus rotundus and Segniliparus rugosus Infection and Analysis of Their Comparative Virulence Profiles
Authors
 Jong-Seok Kim; Woo Sik Kim; Sung Jae Shin; Won-Jung Koh; Seok-Yong Eum; Jin Man Kim; Choul-Jae Won; Keehoon Lee
Issue Date
2013
Journal Title
 PLoS One
ISSN
 1932-6203
Citation
 PLoS One, Vol.8(3) : e59646, 2013
Abstract
Two closely related bacterial species, Segniliparus rotundus and Segniliparus rugosus, have emerged as important human pathogens, but little is known about the immune responses they elicit or their comparative pathophysiologies. To determine the virulence and immune responses of the two species, we compared their abilities to grow in phagocytic and non-phagocytic cells. Both species maintained non-replicating states within A549 epithelial cells. S. rugosus persisted longer and multiplied more rapidly inside murine bone marrow-derived macrophages (BMDMs), induced more pro-inflammatory cytokines, and induced higher levels of macrophage necrosis. Activation of BMDMs by both species was mediated by toll-like receptor 2 (TLR2), followed by mitogen-activated protein kinases (MAPK) and nuclear factor κB (NF-κB) signaling pathways, indicating a critical role for TLR2 in Segniliparus-induced macrophage activation. S. rugosus triggered faster and stronger activation of MAPK signaling and IκB degradation, indicating that S. rugosus induces more pro-inflammatory cytokines than S. rotundus. Multifocal granulomatous inflammations in the liver and lung were observed in mice infected with S. rugosus, but S. rotundus was rapidly cleared from all organs tested within 15 days post-infection. Furthermore, S. rugosus induced faster infiltration of innate immune cells such as neutrophils and macrophages to the lung than S. rotundus. Our results suggest that S. rugosus is more virulent and induces a stronger immune response than S. rotundus.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/86568
DOI
10.1371/journal.pone.0059646
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Microbiology
1. 연구논문 > 5. Research Institutes > Institute for Immunology and Immunological Disease
Yonsei Authors
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