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Ihh and Runx2/Runx3 signaling interact to coordinate early chondrogenesis: a mouse model

Authors
 Eun-Jung Kim  ;  Sung-Won Cho  ;  Jeong-Oh Shin  ;  Min-Jung Lee  ;  Kye-Seong Kim  ;  Han-Sung Jung 
Citation
 PLOS ONE, Vol.8(2) : e55296, 2013 
Journal Title
PLOS ONE
Issue Date
2013
MeSH
Animals ; Antibodies, Monoclonal ; Blotting, Western ; Cell Differentiation/physiology ; Chondrogenesis/physiology* ; Core Binding Factor Alpha 1 Subunit/metabolism* ; Core Binding Factor Alpha 3 Subunit/metabolism* ; DNA Primers/genetics ; Gene Expression Regulation, Developmental/physiology* ; Hedgehog Proteins/metabolism* ; In Situ Hybridization ; Kruppel-Like Transcription Factors/metabolism ; Luciferases ; Mesenchymal Stromal Cells/physiology ; Mice ; Microarray Analysis ; Reverse Transcriptase Polymerase Chain Reaction ; Signal Transduction/physiology* ; X-Ray Microtomography ; Zinc Finger Protein GLI1
Keywords
Animals ; Antibodies, Monoclonal ; Blotting, Western ; Cell Differentiation/physiology ; Chondrogenesis/physiology* ; Core Binding Factor Alpha 1 Subunit/metabolism* ; Core Binding Factor Alpha 3 Subunit/metabolism* ; DNA Primers/genetics ; Gene Expression Regulation, Developmental/physiology* ; Hedgehog Proteins/metabolism* ; In Situ Hybridization ; Kruppel-Like Transcription Factors/metabolism ; Luciferases ; Mesenchymal Stromal Cells/physiology ; Mice ; Microarray Analysis ; Reverse Transcriptase Polymerase Chain Reaction ; Signal Transduction/physiology* ; X-Ray Microtomography ; Zinc Finger Protein GLI1
Abstract
Endochondral bone formation begins with the development of a cartilage intermediate that is subsequently replaced by calcified bone. The mechanisms occurring during early chondrogenesis that control both mesenchymal cell differentiation into chondrocytes and cell proliferation are not clearly understood in vertebrates. Indian hedgehog (Ihh), one of the hedgehog signaling molecules, is known to control both the hypertrophy of chondrocytes and bone replacement; these processes are particularly important in postnatal endochondral bone formation rather than in early chondrogenesis. In this study, we utilized the maternal transfer of 5E1 to E12.5 in mouse embryos, a process that leads to an attenuation of Ihh activity. As a result, mouse limb bud chondrogenesis was inhibited, and an exogenous recombinant IHH protein enhanced the proliferation and differentiation of mesenchymal cells. Analysis of the genetic relationships in the limb buds suggested a more extensive role for Ihh and Runx genes in early chondrogenesis. The transfer of 5E1 decreased the expression of Runx2 and Runx3, whereas an exogenous recombinant IHH protein increased Runx2 and Runx3 expression. Moreover, a transcription factor Gli1 in hedgehog pathway enhances the direct induction of both Runx2 and Runx3 transcription. These findings suggested that Ihh signaling plays an important role in chondrocyte proliferation and differentiation via interactions with Runx2 and Runx3
Files in This Item:
T201300271.pdf Download
DOI
10.1371/journal.pone.0055296
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Eun-Jung(김은정) ORCID logo https://orcid.org/0000-0002-9515-7590
Shin, Jeong Oh(신정오) ORCID logo https://orcid.org/0000-0002-6935-0936
Lee, Min Jung(이민정)
Jung, Han Sung(정한성) ORCID logo https://orcid.org/0000-0003-2795-531X
Cho, Sung Won(조성원) ORCID logo https://orcid.org/0000-0001-7505-9769
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/86302
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