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Dynamic modulation of ANO1/TMEM16A HCO3− permeability by Ca2+/calmodulin

Authors
 Jinsei Jung ; Joo Hyun Nam ; Min Goo Lee ; Joo-Heon Yoon ; Uhtaek Oh ; Hyun Woo Park 
Citation
 Proceedings of the National Academy of Sciences of the United States of America, Vol.110(1) : 360~365, 2013 
Journal Title
 Proceedings of the National Academy of Sciences of the United States of America 
ISSN
 0027-8424 
Issue Date
2013
Abstract
Anoctamin 1 (ANO1)/transmembrane protein 16A (TMEM16A) is a calcium-activated anion channel that may play a role in HCO3− secretion in epithelial cells. Here, we report that the anion selectivity of ANO1 is dynamically regulated by the Ca2+/calmodulin complex. Whole-cell current measurements in HEK 293T cells indicated that ANO1 becomes highly permeable to HCO3− at high [Ca2+]i. Interestingly, this result was not observed in excised patches, indicating the involvement of cytosolic factors in this process. Further studies revealed that the direct association between ANO1 and calmodulin at high [Ca2+]i is responsible for changes in anion permeability. Calmodulin physically interacted with ANO1 in a [Ca2+]i-dependent manner, and addition of recombinant calmodulin to the cytosolic side of excised patches reversibly increased PHCO3/PCl. In addition, the high [Ca2+]i-induced increase in HCO3− permeability was reproduced in mouse submandibular gland acinar cells, in which ANO1 plays a critical role in fluid secretion. These results indicate that the HCO3− permeability of ANO1 can be dynamically modulated and that ANO1 may play an important role in cellular HCO3− transport, especially in transepithelial HCO3− secretion.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/86271
DOI
10.1073/pnas.1211594110
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Pharmacology
1. 연구논문 > 1. College of Medicine > Dept. of Otorhinolaryngology
Yonsei Authors
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