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Identifying genes related to radiation resistance in oral squamous cell carcinoma cell lines

Title
 Identifying genes related to radiation resistance in oral squamous cell carcinoma cell lines 
Authors
 S.Y. Lee ; H.R. Park ; S.-H. Kim ; S.W. Park ; S.Y. Rha ; Y.P. Choi ; N.H. Cho 
Issue Date
2013
Journal Title
 International Journal of Oral and Maxillofacial Surgery 
ISSN
 0901-5027 
Citation
 International Journal of Oral and Maxillofacial Surgery, Vol.42(2) : 169~176, 2013 
Abstract
Radioresistance is one of the main determinants of treatment outcome in oral cancer, but the prediction of radioresistance is difficult. The authors aimed to establish radioresistant oral squamous cell carcinoma (OSCC) cell lines to identify genes with altered expression in response to radioresistance. To induce radioresistant cell lines, the authors treated OSCC cell lines with an accumulated dosage of 60Gy over 30 cycles of radiotherapy. They compared the results from cDNA arrays and proteomics between non-radiated and radioresistant cell lines in order to identify changes in gene expression. Western blot analysis was used to validate the results. The cDNA array revealed 265 commonly up-regulated genes and 268 commonly down-regulated genes in radioresistant cell lines, 30 of which were cancer-related genes. Proteomics identified 51 proteins with commonly altered expression in radioresistant cell lines, 18 of which were cancer-related proteins. Both the cDNA array and proteomics indicated that NM23-H1 and PA2G4 were over-expressed. Western blot analysis showed increased expression of NM23-H1, but not PA2G4, in radioresistant cell lines. The authors concluded that NM23-H1 may be a radioresistance-related gene and over-expression of NM23-H1 could serve as a biomarker to predict radioresistance in OSCC.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/86199
DOI
10.1016/j.ijom.2012.10.022
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Otorhinolaryngology
1. 연구논문 > 1. College of Medicine > Dept. of Pathology
1. 연구논문 > 1. College of Medicine > Yonsei Biomedical Research Center
1. 연구논문 > 1. College of Medicine > Dept. of Internal Medicine
Yonsei Authors
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Link
 http://www.sciencedirect.com/science/article/pii/S0901502712004377
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