Cited 0 times in
Sniper2L is a high-fidelity Cas9 variant with high activity
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김형범 | - |
dc.date.accessioned | 2023-07-12T02:27:06Z | - |
dc.date.available | 2023-07-12T02:27:06Z | - |
dc.date.issued | 2023-03 | - |
dc.identifier.issn | 1552-4450 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/195312 | - |
dc.description.abstract | Although several high-fidelity SpCas9 variants have been reported, it has been observed that this increased specificity is associated with reduced on-target activity, limiting the applications of the high-fidelity variants when efficient genome editing is required. Here, we developed an improved version of Sniper-Cas9, Sniper2L, which represents an exception to this trade-off trend as it showed higher specificity with retained high activity. We evaluated Sniper2L activities at a large number of target sequences and developed DeepSniper, a deep learning model that can predict the activity of Sniper2L. We also confirmed that Sniper2L can induce highly efficient and specific editing at a large number of target sequences when it is delivered as a ribonucleoprotein complex. Mechanically, the high specificity of Sniper2L originates from its superior ability to avoid unwinding a target DNA containing even a single mismatch. We envision that Sniper2L will be useful when efficient and specific genome editing is required. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Nature Pub. Group | - |
dc.relation.isPartOf | NATURE CHEMICAL BIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Sniper2L is a high-fidelity Cas9 variant with high activity | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pharmacology (약리학교실) | - |
dc.contributor.googleauthor | Young-Hoon Kim | - |
dc.contributor.googleauthor | Nahye Kim | - |
dc.contributor.googleauthor | Ikenna Okafor | - |
dc.contributor.googleauthor | Sungchul Choi | - |
dc.contributor.googleauthor | Seonwoo Min | - |
dc.contributor.googleauthor | Joonsun Lee | - |
dc.contributor.googleauthor | Seung-Min Bae | - |
dc.contributor.googleauthor | Keunwoo Choi | - |
dc.contributor.googleauthor | Janice Choi | - |
dc.contributor.googleauthor | Vinayak Harihar | - |
dc.contributor.googleauthor | Youngho Kim | - |
dc.contributor.googleauthor | Jin-Soo Kim | - |
dc.contributor.googleauthor | Benjamin P Kleinstiver | - |
dc.contributor.googleauthor | Jungjoon K Lee | - |
dc.contributor.googleauthor | Taekjip Ha | - |
dc.contributor.googleauthor | Hyongbum Henry Kim | - |
dc.identifier.doi | 10.1038/s41589-023-01279-5 | - |
dc.contributor.localId | A01148 | - |
dc.relation.journalcode | J02292 | - |
dc.identifier.eissn | 1552-4469 | - |
dc.identifier.pmid | 36894722 | - |
dc.identifier.url | https://www.nature.com/articles/s41589-023-01279-5 | - |
dc.contributor.alternativeName | Kim, Hyongbum | - |
dc.contributor.affiliatedAuthor | 김형범 | - |
dc.citation.startPage | epub | - |
dc.identifier.bibliographicCitation | NATURE CHEMICAL BIOLOGY, : epub, 2023-03 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.