Perchloric acid 가용성 Mycobacterium tuberculosis 항원이 말초혈액 림프구 증식에 미치는 영향

Abstract

Mycobacterium tuberculosis has very complex antigenic structures and large number of antigenic determinants. Due to recent development of immunology, the study on the role of each antigen and its mutual response in human immune system by extracting antigens from the acid-fast bacillus have undergone rapid development. Also, certain types of antigen have been studied on how they are related to antibody and cell mediated immune response by revealing molecular structures. In that regard, the authors investigated its antigenicity from such antigen by extract antigen(TB-PBE) using perchloric acid, ethanol, and boiling out of M. tuberculosis. TB-PBE was composed of only one antigenic band, molecular weight of 58kDa. A 58kDa M. tuberculosis antigen(TB-PBE) stimulated peripheral blood lymphocytes of normal adult for a period from the 1st to the 14th at various times(lst, 2nd, 3rd, 4th, 7th, 9th, 10th, 14th day) in various concentrations (10, 1, 0.1, 0.01pg/ml) and after the stimulation, mitogenesis was measured by H-thymidine and measured B cell, T cell, CD4, CD8, DR, y T cell. ELISA was used to measure IL-2 and IL-4. TB-FBE showed the strongest stimulus effect 7 days after the stimulation. After the stimulation, no change in B and T cells was observed but the number of CD3+ and DR+ cells increased and observed more activities of CD4 T cells due to increase in the ratio of CD4/CD8, Only IL-2 could be measured in cultured supernatant and IL-4 was not observed. TB-PBE antigen showed stimulatory effect on CD4 T cells in peripheral lymphocytes and caused more activation of Th1 than Th2. TB-PBE antigen exists on the surface of M, tuberculosis and induces only cellular immunity.