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Cell-based analysis of pairwise interactions between the components of the multi-tRNA synthetase complex

Authors
 Jiwon Kong  ;  Sunghoon Kim 
Citation
 FASEB JOURNAL, Vol.34(8) : 10476-10488, 2020-08 
Journal Title
FASEB JOURNAL
ISSN
 0892-6638 
Issue Date
2020-08
MeSH
Amino Acyl-tRNA Synthetases / metabolism* ; Animals ; CHO Cells ; Cell Line ; Cricetulus ; Interferon-gamma / metabolism ; Mammals / metabolism ; Protein Interaction Maps / physiology ; Ribosomes / metabolism
Keywords
aminoacyl-tRNA synthetase ; macromolecular complex ; mammalian multi-tRNA synthetase complex ; ribosome ; tRNA
Abstract
Cytoplasmic aminoacyl-tRNA synthetases (ARSs) are organized into multi-tRNA synthetase complexes (MSCs), from Archaea to mammals. An evolutionary conserved role of the MSCs is enhancement of aminoacylation by forming stable associations of the ARSs and tRNAs. In mammals, a single macromolecular MSC exists, which is composed of eight cytoplasmic ARSs, for nine amino acids, and three scaffold proteins. Consequently, nearly half of aminoacyl-tRNA efflux becomes concentrated at the MSC. Stable supply of aminoacyl-tRNA to the ribosome is, therefore, considered to be a major role of the mammalian MSC. Furthermore, the mammalian MSC also serves as a reservoir for releasable components with noncanonical functions. In this study, a split-luciferase complementation system was applied to investigate the configuration of the MSC in live mammalian cells. Multiplex interconnections between the components were simplified into binary protein-protein interactions, and pairwise comparison of the interactions reconstituted a framework consistent with previous in vitro studies. Reversibility of the split-luciferase reporter binding demonstrated convertible organization of the mammalian MSC, including interferon gamma (IFNγ)-stimulated glutamyl-prolyl-tRNA synthetase 1 (EPRS1) release, as well as the cooperation with the ribosome bridged by the tRNAs. The cell-based analysis provided an improved understanding of the flexible framework of the mammalian MSC in physiological conditions.
Full Text
https://faseb.onlinelibrary.wiley.com/doi/10.1096/fj.202000418R
DOI
10.1096/fj.202000418R
Appears in Collections:
1. College of Medicine (의과대학) > Others (기타) > 1. Journal Papers
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/194846
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