Cited 6 times in
Magnetofluoro-Immunosensing Platform Based on Binary Nanoparticle-Decorated Graphene for Detection of Cancer Cell-Derived Exosomes
DC Field | Value | Language |
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dc.contributor.author | 김한상 | - |
dc.date.accessioned | 2022-12-22T03:17:35Z | - |
dc.date.available | 2022-12-22T03:17:35Z | - |
dc.date.issued | 2022-08 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/191836 | - |
dc.description.abstract | Multi-functionalized carbon nanomaterials have attracted interest owing to their excellent synergic properties, such as plasmon resonance energy transfer and surface-enhanced Raman scattering. Particularly, nanoparticle (NP)-decorated graphene (GRP) has been applied in various fields. In this study, silver NP (AgNP)- and magnetic iron oxide NP (IONP)-decorated GRP were prepared and utilized as biosensing platforms. In this case, AgNPs and GRP exhibit plasmonic properties, whereas IONPs exhibit magnetic properties; therefore, this hybrid nanomaterial could function as a magnetoplasmonic substrate for the magnetofluoro-immunosensing (MFI) system. Conversely, exosomes were recently considered high-potential biomarkers for the diagnosis of diseases. However, exosome diagnostic use requires complex isolation and purification methods. Nevertheless, we successfully detected a prostate-cancer-cell-derived exosome (PC-exosome) from non-purified exosomes in a culture media sample using Ag/IO-GRP and dye-tetraspanin antibodies (Ab). First, the anti-prostate-specific antigen was immobilized on the Ag/IO-GRP and it could isolate the PC-exosome from the sample via an external magnetic force. Dye-tetraspanin Ab was added to the sample to induce the sandwich structure. Based on the number of exosomes, the fluorescence intensity from the dye varied and the system exhibited highly sensitive and selective performance. Consequently, these hybrid materials exhibited excellent potential for biosensing platforms. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Biosensing Techniques* / methods | - |
dc.subject.MESH | Exosomes* | - |
dc.subject.MESH | Gold / chemistry | - |
dc.subject.MESH | Graphite* / chemistry | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Nanoparticles* / chemistry | - |
dc.subject.MESH | Neoplasms* | - |
dc.title | Magnetofluoro-Immunosensing Platform Based on Binary Nanoparticle-Decorated Graphene for Detection of Cancer Cell-Derived Exosomes | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.googleauthor | Jaewook Lee | - |
dc.contributor.googleauthor | Ji-Heon Lee | - |
dc.contributor.googleauthor | Jagannath Mondal | - |
dc.contributor.googleauthor | Joon Hwang | - |
dc.contributor.googleauthor | Han Sang Kim | - |
dc.contributor.googleauthor | Vinoth Kumar | - |
dc.contributor.googleauthor | Akhil Raj | - |
dc.contributor.googleauthor | Seung Rim Hwang | - |
dc.contributor.googleauthor | Yong-Kyu Lee | - |
dc.identifier.doi | 10.3390/ijms23179619 | - |
dc.contributor.localId | A01098 | - |
dc.relation.journalcode | J01133 | - |
dc.identifier.eissn | 1422-0067 | - |
dc.identifier.pmid | 36077015 | - |
dc.subject.keyword | binary nanoparticle-decorated graphene | - |
dc.subject.keyword | cancer diagnosis | - |
dc.subject.keyword | cancer-derived exosome | - |
dc.subject.keyword | magnetofluoro-immunoassay | - |
dc.subject.keyword | magnetoplasmonic graphene | - |
dc.subject.keyword | prostate cancer | - |
dc.contributor.alternativeName | Kim, Han Sang | - |
dc.contributor.affiliatedAuthor | 김한상 | - |
dc.citation.volume | 23 | - |
dc.citation.number | 17 | - |
dc.citation.startPage | 9619 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, Vol.23(17) : 9619, 2022-08 | - |
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