Cited 2 times in
Dll4 Inhibition Promotes Graft Retention in Fat Grafting Enriched with Adipose-Derived Stem Cells
DC Field | Value | Language |
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dc.contributor.author | 이충근 | - |
dc.date.accessioned | 2022-12-22T02:52:39Z | - |
dc.date.available | 2022-12-22T02:52:39Z | - |
dc.date.issued | 2022-07 | - |
dc.identifier.issn | 2157-6564 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/191722 | - |
dc.description.abstract | Autologous fat grafting is among the safest and most effective treatments for soft-tissue restoration and augmentation, and many efforts have been made to improve its efficiency, including adipose-derived stem cell (ASC) supplementation. Here, we investigated the role of Notch ligand Delta-like ligand 4 (Dll4) in angiogenesis within grafted fat and its effect on graft retention, as well as the effect of Dll4 inhibition on ASC supplementation. Using a murine fat graft model, we investigated the expression of Dll4 in fat grafts and assessed the graft volume, vascularity, and perfusion within the graft, and ASC differentiation patterns depending on the blockade of Dll4. The underlying mechanism of Dll4 inhibition on ASC supplemented fat grafts was investigated using transcriptome analysis. Dll4 was highly expressed in vascular endothelial cells (ECs) within grafted fat, where Dll4-blocking antibody treatment-induced angiogenesis, promoting fat graft retention. In addition, its effect on fat graft retention was synergistically improved when ASCs were concomitantly supplemented. The expression of junctional proteins was increased in ECs, and inflammatory processes were downregulated in grafted fat upon ASC supplementation and Dll4 inhibition. Dll4 inhibition induced vascularization within the grafted fat, thereby promoting graft retention and exhibiting synergistic effects with concomitant ASC supplementation. This study serves as a basis for developing new potential therapeutic approaches targeting Dll4 to improve graft retention after cell-assisted transfer. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | AlphaMed Press. | - |
dc.relation.isPartOf | STEM CELLS TRANSLATIONAL MEDICINE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Adaptor Proteins, Signal Transducing* / physiology | - |
dc.subject.MESH | Adipose Tissue* / transplantation | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Calcium-Binding Proteins* / physiology | - |
dc.subject.MESH | Endothelial Cells* | - |
dc.subject.MESH | Graft Survival* / physiology | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Stem Cells | - |
dc.title | Dll4 Inhibition Promotes Graft Retention in Fat Grafting Enriched with Adipose-Derived Stem Cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.googleauthor | Choong-Kun Lee | - |
dc.contributor.googleauthor | Bo-Yoon Park | - |
dc.contributor.googleauthor | Taehee Jo | - |
dc.contributor.googleauthor | Cheol-Heum Park | - |
dc.contributor.googleauthor | Ju-Hee Kim | - |
dc.contributor.googleauthor | Kyu-Jin Chung | - |
dc.contributor.googleauthor | Yong-Ha Kim | - |
dc.contributor.googleauthor | Do Young Park | - |
dc.contributor.googleauthor | Il-Kug Kim | - |
dc.identifier.doi | 10.1093/stcltm/szac034 | - |
dc.contributor.localId | A03259 | - |
dc.relation.journalcode | J02686 | - |
dc.identifier.eissn | 2157-6580 | - |
dc.identifier.pmid | 35579982 | - |
dc.subject.keyword | adipose tissue | - |
dc.subject.keyword | angiogenesis | - |
dc.subject.keyword | delta-like ligand 4 | - |
dc.subject.keyword | stem cells | - |
dc.subject.keyword | transplantation | - |
dc.contributor.alternativeName | Lee, Choong-kun | - |
dc.contributor.affiliatedAuthor | 이충근 | - |
dc.citation.volume | 11 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 742 | - |
dc.citation.endPage | 752 | - |
dc.identifier.bibliographicCitation | STEM CELLS TRANSLATIONAL MEDICINE, Vol.11(7) : 742-752, 2022-07 | - |
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