Intraoperative somatosensory evoked potentials (SSEPs) play a role in reducing postoperative complication. The method of obtaining the SSEP
of both upper and lower limbs includes not only a method obtained by serially stimulating each limb, but also an interleave method using
auto-sequence. We retrospectively reviewed the clinical and intraoperative monitoring records of 15 patients who underwent both upper and
lower limbs intraoperative SSEPs at our hospital from March to September 2020. We compared the amplitude, latency, and acquisition time
of the results in each patient when stimulated in serial mode and interleave mode, respectively. Interleave mode stimulation showed no difference in latency, greater amplitude, and shorter acquisition time compared to serial mode stimulation. There were no postoperative
complications in 15 patients who underwent intraoperative SSEPs with interleave mode stimulation. Interleave stimulation using auto-sequence can be a clinically useful method for monitoring intraoperative SSEPs of both upper and lower limbs.