알레르기 질환 환자에서 고양이털 항원에 대한 감작을 및 고양이털의 항원성에 관한 연구

Abstract

Objectives: The cat fur allergen used in skin prick test was known to be contaminated with housedust mites. So we intended to evaluate the clinical importance of cat fur as a causative allergen in patients with allergic diseases. Methods: We performed skin prick tests with cat allergen of Torii company(Japan), Bencard company (England) and home made extracts in 387 patients, We detected specific IgE by 3M FAST-plus and ELISA method using home made cat fur allergen, developed at this class. To characterize the allergenic components, cat fur extracts were fractionated by 12 % sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE) and then transferred onto nitrocellulose membrane and probed with sera of catfur sensitive patients Results: 1) Out of 387 patients, 77(19.9%) showed more than 1+results on skin prick test. There were no significant differences in the rates of positive results ac- cording to the allergic diseases, but the age groups were correlated with the positive results(younger than 30: 34.5%, between 30 and 50: 14.4%, older than 50: 6.8%). 2) Cat ELISA showed specific inhibition to cat fur allergen and Cat ELISA inhibition test revealed the linear dose response curve on the added amounts of cat allergn into pooled sera. 67.7% out of Torii cat fur sensitive patients(A/H ratio≥1) showed positive results in Cat ELISA for specific IgE antibody. 3) Cat fur extracts showed 15 protein bands by SDS-PAGE, then they were transferred onto nitrocellulose membrane and probed with sera of 16 cat-fur sensitive patients, one sera pool of patients with RAST class 3, and three negative controls and with biotin-conjugated anti IgE-avidin peroxidase. Six IgE binding protein bands were detected at 94, 73, 56, 44, 28 and 21 kilodalton of molecular weight. Out of six IgE binding bands, MW 21KD concerned as major cat allergen, Fel d 1, were noted in five sera(31.3% ). Conclusion: It is suggested that cat fur can be regarded as an important allergenic fur and that we need further studies to st.andardize cat antigens for in vivo and in vitro tests.