노란털깔다구 항원에 대한 아토피 환아의 면역반응에 관한 연구

Abstract

Hypersensitivity to chironomidas(non biting midge) has been comrnonly found in many countries. To evaluate the role of chironomidae as an inhalant allergen in childhood atopic patients, we performed allergy skin test to crude extracts of Chironomidae flaviplumus(CF) in 456 patients. Positive skin reactions were noted in 35 out of 456(7.8 % ) atopic patients. All of the positive reactors also showed positive responses to other inhalant allergens, especially Dermat- ophagoides farinae. Specific IgE to CF antigens by ELISA were detected in 3 out of 8 patients and specific IgG and IgG subclasses were noted in 8 patients with very low absorbance value. SDS PAGE analysis of CF extracts contains at least 16 antigenic components and 15 IgE- binding components were noted by immunoblotting analysis. Among these allergenic components, three components of 32,000, 27,000, 19,000 dalton are bound strongly with the sera of atopic patients tested. In conclusion, there was a low sensitization rates of CF antigen and low detection rate of CF-specific IgE and IgG. Further studies are needed to investigate its importance as an inhalant allergen in childhood patients and the pathogenic role of specific IgE and IgG antibodies to CF sntigens.