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Isolation of perchloric acid soluble, heat stable, ethanol extractable protein from Mycobacterium tuberculosis
DC Field | Value | Language |
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dc.contributor.author | 김동수 | - |
dc.date.accessioned | 2021-09-28T08:25:47Z | - |
dc.date.available | 2021-09-28T08:25:47Z | - |
dc.date.issued | 1996-10 | - |
dc.identifier.issn | 0513-5796 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/183638 | - |
dc.description.abstract | The perchloric acid soluble, heat stable, and ethanol insoluble antigen of M. tuberculosis (TB-PBE) was prepared, and antigenicity of this antigen was studied in vivo and in vitro. TB-PBE showed a single band of 60 kDa by SDS-PAGE. Sera from the patients with active pulmonary tuberculosis did not react with this antigen by ELISA. A delayed hypersensitivity skin reaction was induced with this antigen and was correlated with the reaction with PPD. Skin biopsy was performed in this skin lesion induced by TB-PBE and stained by H-E and immunohistochemical methods. TB-PBE induced an inflammatory lesion similar to a lesion induced by PPD. Blastogenic activity of the peripheral blood mononuclear cells stimulated by TB-PBE increased, and showed a peak reaction at 7 days after stimulation. The blastogenic activity changed in a dose-dependent manner. After stimulation with TB-PBE, mononuclear cells were analyzed by FACS. DR+ T cells and CD4/CD8 ratio increased after stimulation by TB-PBE. These cells secreted IL-2, not IL-4 after stimulation with TB-PBE. In the immunofluorescence test, mouse antiserum against TB-PBE showed a positive reaction with M. tuberculosis and showed cross-reactivity with M. bovive and other atypical mycobacteria, but not with S. aureus. With these results, it is evident that TB-PBE is an antigen which can induce cell mediated immunity in vivo and in vitro. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Yonsei University | - |
dc.relation.isPartOf | YONSEI MEDICAL JOURNAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Antigens, Bacterial / immunology | - |
dc.subject.MESH | Antigens, Bacterial / isolation & purification* | - |
dc.subject.MESH | Cytokines / biosynthesis | - |
dc.subject.MESH | Electrophoresis, Polyacrylamide Gel | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Hypersensitivity, Delayed | - |
dc.subject.MESH | Lymphocyte Activation | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Inbred BALB C | - |
dc.subject.MESH | Mycobacterium tuberculosis / immunology* | - |
dc.subject.MESH | Perchlorates | - |
dc.subject.MESH | Skin / pathology | - |
dc.subject.MESH | T-Lymphocytes / immunology | - |
dc.title | Isolation of perchloric acid soluble, heat stable, ethanol extractable protein from Mycobacterium tuberculosis | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pediatrics (소아과학교실) | - |
dc.contributor.googleauthor | Dong Soo Kim | - |
dc.contributor.googleauthor | Bu Hyun Han | - |
dc.contributor.googleauthor | Ki Young Lee | - |
dc.identifier.doi | 10.3349/ymj.1996.37.5.339 | - |
dc.contributor.localId | A00405 | - |
dc.relation.journalcode | J02813 | - |
dc.identifier.eissn | 1976-2437 | - |
dc.identifier.pmid | 8997166 | - |
dc.contributor.alternativeName | Kim, Dong Soo | - |
dc.contributor.affiliatedAuthor | 김동수 | - |
dc.citation.volume | 37 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 339 | - |
dc.citation.endPage | 349 | - |
dc.identifier.bibliographicCitation | YONSEI MEDICAL JOURNAL, Vol.37(5) : 339-349, 1996-10 | - |
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