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Labeling-free detection of ECD-HER2 protein using aptamer-based nano-plasmonic sensor
DC Field | Value | Language |
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dc.contributor.author | 서진석 | - |
dc.contributor.author | 양재문 | - |
dc.date.accessioned | 2020-12-01T18:00:46Z | - |
dc.date.available | 2020-12-01T18:00:46Z | - |
dc.date.issued | 2020-04 | - |
dc.identifier.issn | 0957-4484 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/180553 | - |
dc.description.abstract | A gold nanoparticle-based localized surface plasmon resonance substrate has been developed as nano-sensors for various bio-applications. However, reproducible and robust sensing substrates anchored gold nanoparticles has not yet been explored. In this study, dopamine-coated gold nanorods (DGNRs) were prepared and immobilized onto the micro-grooving PDMS substrates (mgPDMS). Subsequently, HER2-specific aptamers were conjugated with DGNR/mgPDMS for ECD-HER2 detection. By screening of the optimal concentration of DGNR and aptamers, the effective HER2-specific aptasensor was built up. In particular, the real-time binding assay for the evaluation of limit-of-detection (<5 ng ml-1) was conducted. Furthermore, the binding kinetics for ECD-HER2 was investigated under the biological fluid using a rat serum. Our HER2-specific aptasensor demonstrated the effective sensitivity and selectivity for ECD-HER2. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | IOP Pub. | - |
dc.relation.isPartOf | NANOTECHNOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Labeling-free detection of ECD-HER2 protein using aptamer-based nano-plasmonic sensor | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Radiology (영상의학교실) | - |
dc.contributor.googleauthor | Jung-Hoon Kim | - |
dc.contributor.googleauthor | Jin-Suck Suh | - |
dc.contributor.googleauthor | Jaemoon Yang | - |
dc.identifier.doi | 10.1088/1361-6528/ab68fa | - |
dc.contributor.localId | A01916 | - |
dc.contributor.localId | A02315 | - |
dc.relation.journalcode | J02287 | - |
dc.identifier.eissn | 1361-6528 | - |
dc.identifier.pmid | 31914433 | - |
dc.identifier.url | https://iopscience.iop.org/article/10.1088/1361-6528/ab68fa | - |
dc.subject.keyword | Animals | - |
dc.subject.keyword | Aptamers, Nucleotide / chemistry* | - |
dc.subject.keyword | Biosensing Techniques | - |
dc.subject.keyword | Dopamine / chemistry* | - |
dc.subject.keyword | Gold | - |
dc.subject.keyword | Limit of Detection | - |
dc.subject.keyword | Male | - |
dc.subject.keyword | Metal Nanoparticles | - |
dc.subject.keyword | Rats | - |
dc.subject.keyword | Receptor, ErbB-2 / analysis* | - |
dc.subject.keyword | Receptor, ErbB-2 / blood | - |
dc.subject.keyword | Surface Plasmon Resonance | - |
dc.contributor.alternativeName | Suh, Jin Suck | - |
dc.contributor.affiliatedAuthor | 서진석 | - |
dc.contributor.affiliatedAuthor | 양재문 | - |
dc.citation.volume | 31 | - |
dc.citation.number | 17 | - |
dc.citation.startPage | 175501 | - |
dc.identifier.bibliographicCitation | NANOTECHNOLOGY, Vol.31(17) : 175501, 2020-04 | - |
dc.identifier.rimsid | 67450 | - |
dc.type.rims | ART | - |
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