Cited 0 times in
Helicobater pylori에 의한 위암세포주 사멸
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김원호 | - |
dc.contributor.author | 여말희 | - |
dc.contributor.author | 이경원 | - |
dc.contributor.author | 이용찬 | - |
dc.date.accessioned | 2020-01-10T02:08:29Z | - |
dc.date.available | 2020-01-10T02:08:29Z | - |
dc.date.issued | 1999 | - |
dc.identifier.issn | 1598-9992 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/173902 | - |
dc.description.abstract | Background/Aims: Helicobacter pylori (H. pylori) is associated with active gastritis and peptic ulcer disease. Mechanism for H. pylori-induced gastric epithelial damage is still incompletely understood. However, the increase of apoptotic cells in H. pylori-infected mucosa suggested that apoptosis could be a major mechanism for cellular damage. As an effort to clarify the mechanism, we investigated whether H. pylori directly induce apoptosis in gastric cancer cells in vitro. Methods: Cultured H. pylori (ATCC 43504) were suspended as 109/mL. IL (interleukin)-8 was measured by enzyme linked immunosorbent assay. Cell survival was assessed by MTT [3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. Apoptosis was detected and confirmed by demonstration of DNA fragmentation and morphologic changes. Results: H. pylori induced IL-8 production as well as decrease of cell survival in gastric cancer cell lines in a time- and concentration-dependen way. Addition of H. pylori to gastric cancer cells induced apoptosis. Such induction was not organ specific. Heat or formalin treatment of H. pylori almost completely inhibited IL-8 production but only partially blocked apoptosis. H. pylori- induced apoptosis was potentiated by interferon-γ pretreatment in HT-29 but not in AGS and KATO III. Conclusions: These results suggest that H. pylori affects on gastric epithelial cell growth by direct induction of apoptosis. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | Korean Journal of Gastroenterology | - |
dc.publisher | Korean Journal of Gastroenterology | - |
dc.relation.isPartOf | Korean Journal of Gastroenterology | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Helicobater pylori에 의한 위암세포주 사멸 | - |
dc.title.alternative | Helicobacter pylori-induced Apoptosis in Gastric Cancer Cell Lines | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.googleauthor | 김원호 | - |
dc.contributor.googleauthor | 여말희 | - |
dc.contributor.googleauthor | 이꽃실 | - |
dc.contributor.googleauthor | 이용찬 | - |
dc.contributor.googleauthor | 박인서 | - |
dc.contributor.googleauthor | 이경원 | - |
dc.contributor.localId | A00774 | - |
dc.contributor.localId | A02345 | - |
dc.contributor.localId | A02649 | - |
dc.contributor.localId | A02988 | - |
dc.relation.journalcode | J02015 | - |
dc.identifier.eissn | 2233-6869 | - |
dc.subject.keyword | Helicobacter pylori | - |
dc.subject.keyword | Apoptosis | - |
dc.subject.keyword | Interleukin-8 | - |
dc.contributor.alternativeName | Kim, Won Ho | - |
dc.contributor.affiliatedAuthor | 김원호 | - |
dc.contributor.affiliatedAuthor | 여말희 | - |
dc.contributor.affiliatedAuthor | 이경원 | - |
dc.contributor.affiliatedAuthor | 이용찬 | - |
dc.citation.volume | 34 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 21 | - |
dc.citation.endPage | 34 | - |
dc.identifier.bibliographicCitation | Korean Journal of Gastroenterology, Vol.34(1) : 21-34, 1999 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.